KOPS - The Institutional Repository of the University of Konstanz

Chemical proteomics reveals interactors of the alarmone diadenosine triphosphate in the cancer cell line H1299

Chemical proteomics reveals interactors of the alarmone diadenosine triphosphate in the cancer cell line H1299

Cite This

Files in this item

Files Size Format View

There are no files associated with this item.

ALBRECHT, Christoph J, Florian M STUMPF, Lena KRÜGER, Marie L NIEDERMEIER, Florian STENGEL, Andreas MARX, 2022. Chemical proteomics reveals interactors of the alarmone diadenosine triphosphate in the cancer cell line H1299. In: Journal of Peptide Science. Wiley. ISSN 1075-2617. eISSN 1099-1387. Available under: doi: 10.1002/psc.3458

@article{Albrecht2022-10-20Chemi-59109, title={Chemical proteomics reveals interactors of the alarmone diadenosine triphosphate in the cancer cell line H1299}, year={2022}, doi={10.1002/psc.3458}, issn={1075-2617}, journal={Journal of Peptide Science}, author={Albrecht, Christoph J and Stumpf, Florian M and Krüger, Lena and Niedermeier, Marie L and Stengel, Florian and Marx, Andreas} }

Stumpf, Florian M Marx, Andreas Marx, Andreas Krüger, Lena eng Stengel, Florian Chemical proteomics reveals interactors of the alarmone diadenosine triphosphate in the cancer cell line H1299 2022-10-20 Krüger, Lena 2022-11-10T14:08:19Z Albrecht, Christoph J Niedermeier, Marie L Niedermeier, Marie L Intracellular dinucleoside polyphosphates (Np<sub>n</sub> Ns) have been known for decades but the functional role remains enigmatic. Diadenosine triphosphate (Ap<sub>3</sub> A) is one of the most prominent examples, and its intercellular concentration was shown to increase upon cellular stress. By employment of previously reported Ap3 A-based photoaffinity-labeling probes (PALPs) in chemical proteomics, we investigated the Ap3 A interactome in the human lung carcinoma cell line H1299. The cell line is deficient of the fragile histidine triade (Fhit) protein, a hydrolase of Ap<sub>3</sub> A and tumor suppressor. Overall, the number of identified potential interaction partners was significantly lower than in the previously investigated HEK293T cell line. Gene ontology term analysis revealed that the identified proteins participate in similar pathways as for HEK293T, but the percentage of proteins involved in RNA-related processes is higher for H1299. The obtained results highlight similarities and differences of the Ap3 A interaction network in different cell lines and give further indications regarding the importance of the presence of Fhit. Attribution 4.0 International Albrecht, Christoph J Stengel, Florian Stumpf, Florian M 2022-11-10T14:08:19Z

This item appears in the following Collection(s)

Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International

Search KOPS


Browse

My Account