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Extracellular Gelsolin Binds Lipoteichoic Acid and Modulates Cellular Response to Proinflammatory Bacterial Wall Components

Extracellular Gelsolin Binds Lipoteichoic Acid and Modulates Cellular Response to Proinflammatory Bacterial Wall Components

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BUCKI, Robert, Fitzroy J. BYFIELD, Alina KULAKOWSKA, Margaret E. MCCORMICK, Wieslaw DROZDOWSKI, Zbigniew NAMIOT, Thomas HARTUNG, Paul A. JANMEY, 2008. Extracellular Gelsolin Binds Lipoteichoic Acid and Modulates Cellular Response to Proinflammatory Bacterial Wall Components. In: The Journal of Immunology. American Association of Immunologist. 181(7), pp. 4936-4944. ISSN 0022-1767. eISSN 1550-6606. Available under: doi: 10.4049/jimmunol.181.7.4936

@article{Bucki2008Extra-58693, title={Extracellular Gelsolin Binds Lipoteichoic Acid and Modulates Cellular Response to Proinflammatory Bacterial Wall Components}, year={2008}, doi={10.4049/jimmunol.181.7.4936}, number={7}, volume={181}, issn={0022-1767}, journal={The Journal of Immunology}, pages={4936--4944}, author={Bucki, Robert and Byfield, Fitzroy J. and Kulakowska, Alina and McCormick, Margaret E. and Drozdowski, Wieslaw and Namiot, Zbigniew and Hartung, Thomas and Janmey, Paul A.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/58693"> <dc:contributor>Byfield, Fitzroy J.</dc:contributor> <dcterms:abstract xml:lang="eng">The various functions of gelsolin in extracellular compartments are not yet clearly defined but include actin scavenging and antiinflammatory effects. Gelsolin was recently reported to bind endotoxin (LPS) from various Gram-negative bacteria with high affinity. In this study we investigate whether gelsolin also interacts with bacterial wall molecules of Gram-positive bacteria such as lipoteichoic acid (LTA) and whether gelsolin’s interaction with bacterial lipids from Gram-negative or Gram-positive bacteria affects their cellular inflammatory responses. A peptide based on the PPI binding site of gelsolin (160–169) binds purified LTA at the same molecular ratio that it binds phosphatidylinositol 4,5-bisphosphate. The OD of recombinant human plasma gelsolin was found to decrease following the addition of purified LTA, and the binding of gelsolin to LTA inhibits F-actin depolymerization by gelsolin. Simultaneously, the ability of LTA to activate translocation of NF-κB, E-selectin expression, and adhesion of neutrophils to LTA-treated human aortic endothelial cells was compromised by gelsolin. Gelsolin was able to partially inhibit LPS- or LTA-induced release of IL-8 from human neutrophils but was unable to prevent Gram-positive Bacillus subtilis or Gram-negative Pseudomonas aeruginosa growth and had no effect on the antibacterial activity of the cathelicidin-derived antibacterial peptide LL37. These data suggest that extracellular gelsolin is involved in the host immune recognition of LTA or LPS following release of these molecules from the bacterial outer membrane during cell division or attack by drugs and immune components.</dcterms:abstract> <dc:creator>Namiot, Zbigniew</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/58693"/> <dc:contributor>Drozdowski, Wieslaw</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Byfield, Fitzroy J.</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Bucki, Robert</dc:creator> <dc:creator>Hartung, Thomas</dc:creator> <dc:creator>Kulakowska, Alina</dc:creator> <dc:creator>Janmey, Paul A.</dc:creator> <dc:contributor>Janmey, Paul A.</dc:contributor> <dc:creator>McCormick, Margaret E.</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-09-27T12:22:31Z</dcterms:available> <dc:language>eng</dc:language> <dcterms:title>Extracellular Gelsolin Binds Lipoteichoic Acid and Modulates Cellular Response to Proinflammatory Bacterial Wall Components</dcterms:title> <dc:contributor>McCormick, Margaret E.</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Bucki, Robert</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-09-27T12:22:31Z</dc:date> <dc:contributor>Namiot, Zbigniew</dc:contributor> <dc:creator>Drozdowski, Wieslaw</dc:creator> <dc:contributor>Kulakowska, Alina</dc:contributor> <dc:contributor>Hartung, Thomas</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:issued>2008</dcterms:issued> </rdf:Description> </rdf:RDF>

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