Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1
Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1
Date
2022
Authors
Prattes, Michael
Grishkovskaya, Irina
Hodirnau, Victor-Valentin
Hetzmannseder, Christina
Zisser, Gertrude
Kargas, Vasileios
Loibl, Mathias
Gerhalter, Magdalena
Kofler, Lisa
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
DOI (citable link)
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Title in another language
Publication type
Journal article
Publication status
Published
Published in
Nature Structural & Molecular Biology ; 2022. - Nature Publishing Group. - ISSN 1545-9993. - eISSN 1545-9985
Abstract
The AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis that initiates cytoplasmic maturation of the large ribosomal subunit. Drg1 releases the shuttling maturation factor Rlp24 from pre-60S particles shortly after nuclear export, a strict requirement for downstream maturation. The molecular mechanism of release remained elusive. Here, we report a series of cryo-EM structures that captured the extraction of Rlp24 from pre-60S particles by Saccharomyces cerevisiae Drg1. These structures reveal that Arx1 and the eukaryote-specific rRNA expansion segment ES27 form a joint docking platform that positions Drg1 for efficient extraction of Rlp24 from the pre-ribosome. The tips of the Drg1 N domains thereby guide the Rlp24 C terminus into the central pore of the Drg1 hexamer, enabling extraction by a hand-over-hand translocation mechanism. Our results uncover substrate recognition and processing by Drg1 step by step and provide a comprehensive mechanistic picture of the conserved modus operandi of AAA-ATPases.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
PRATTES, Michael, Irina GRISHKOVSKAYA, Victor-Valentin HODIRNAU, Christina HETZMANNSEDER, Gertrude ZISSER, Carolin SAILER, Vasileios KARGAS, Mathias LOIBL, Magdalena GERHALTER, Lisa KOFLER, Alan J. WARREN, Florian STENGEL, David HASELBACH, Helmut BERGLER, 2022. Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1. In: Nature Structural & Molecular Biology. Nature Publishing Group. ISSN 1545-9993. eISSN 1545-9985. Available under: doi: 10.1038/s41594-022-00832-5BibTex
@article{Prattes2022Visua-58669, year={2022}, doi={10.1038/s41594-022-00832-5}, title={Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1}, issn={1545-9993}, journal={Nature Structural & Molecular Biology}, author={Prattes, Michael and Grishkovskaya, Irina and Hodirnau, Victor-Valentin and Hetzmannseder, Christina and Zisser, Gertrude and Sailer, Carolin and Kargas, Vasileios and Loibl, Mathias and Gerhalter, Magdalena and Kofler, Lisa and Warren, Alan J. and Stengel, Florian and Haselbach, David and Bergler, Helmut} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/58669"> <dc:contributor>Gerhalter, Magdalena</dc:contributor> <dc:contributor>Haselbach, David</dc:contributor> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/58669/1/Prattes_2-19g8038mjcmki6.pdf"/> <dc:creator>Zisser, Gertrude</dc:creator> <dc:creator>Sailer, Carolin</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:creator>Haselbach, David</dc:creator> <dc:creator>Stengel, Florian</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/58669/1/Prattes_2-19g8038mjcmki6.pdf"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:language>eng</dc:language> <dc:contributor>Grishkovskaya, Irina</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/58669"/> <dc:contributor>Kargas, Vasileios</dc:contributor> <dcterms:abstract xml:lang="eng">The AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis that initiates cytoplasmic maturation of the large ribosomal subunit. Drg1 releases the shuttling maturation factor Rlp24 from pre-60S particles shortly after nuclear export, a strict requirement for downstream maturation. The molecular mechanism of release remained elusive. Here, we report a series of cryo-EM structures that captured the extraction of Rlp24 from pre-60S particles by Saccharomyces cerevisiae Drg1. These structures reveal that Arx1 and the eukaryote-specific rRNA expansion segment ES27 form a joint docking platform that positions Drg1 for efficient extraction of Rlp24 from the pre-ribosome. The tips of the Drg1 N domains thereby guide the Rlp24 C terminus into the central pore of the Drg1 hexamer, enabling extraction by a hand-over-hand translocation mechanism. Our results uncover substrate recognition and processing by Drg1 step by step and provide a comprehensive mechanistic picture of the conserved modus operandi of AAA-ATPases.</dcterms:abstract> <dc:contributor>Kofler, Lisa</dc:contributor> <dc:rights>Attribution 4.0 International</dc:rights> <dc:contributor>Hetzmannseder, Christina</dc:contributor> <dc:creator>Grishkovskaya, Irina</dc:creator> <dc:creator>Hetzmannseder, Christina</dc:creator> <dc:creator>Gerhalter, Magdalena</dc:creator> <dc:contributor>Zisser, Gertrude</dc:contributor> <dc:contributor>Loibl, Mathias</dc:contributor> <dc:contributor>Prattes, Michael</dc:contributor> <dc:creator>Loibl, Mathias</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Prattes, Michael</dc:creator> <dcterms:title>Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-09-21T10:13:32Z</dcterms:available> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:contributor>Sailer, Carolin</dc:contributor> <dc:contributor>Warren, Alan J.</dc:contributor> <dc:contributor>Stengel, Florian</dc:contributor> <dc:contributor>Hodirnau, Victor-Valentin</dc:contributor> <dc:creator>Kofler, Lisa</dc:creator> <dc:creator>Warren, Alan J.</dc:creator> <dc:contributor>Bergler, Helmut</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-09-21T10:13:32Z</dc:date> <dc:creator>Hodirnau, Victor-Valentin</dc:creator> <dc:creator>Bergler, Helmut</dc:creator> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <dcterms:issued>2022</dcterms:issued> <dc:creator>Kargas, Vasileios</dc:creator> </rdf:Description> </rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
Yes
Refereed
Yes