Comparative ontogenetic and transcriptomic analyses shed light on color pattern divergence in cichlid fishes

Kratochwil, Claudius F.; Liang, Yipeng; Gerwin, Jan; Franchini, Paolo; Meyer, Axel

Comparative ontogenetic and transcriptomic analyses shed light on color pattern divergence in cichlid fishes

Dateien

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Datum

2022

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Evolution & Development. Wiley-Blackwell - STM. 2022, 24(5), pp. 158-170. ISSN 1520-541X. eISSN 1525-142X. Available under: doi: 10.1111/ede.12416

Zusammenfassung

Stripe patterns are a striking example for a repeatedly evolved color pattern. In the African adaptive radiations of cichlid fishes, stripes evolved several times independently. Previously, it has been suggested that regulatory evolution of a single gene, agouti-related-peptide 2 (agrp2), explains the evolutionary lability of this trait. Here, using a comparative transcriptomic approach, we performed comparisons between (adult) striped and nonstriped cichlid fishes of representatives of Lake Victoria and the two major clades of Lake Malawi (mbuna and non-mbuna lineage). We identify agrp2 to be differentially expressed across all pairwise comparisons, reaffirming its association with stripe pattern divergence. We therefore also provide evidence that agrp2 is associated with the loss of the nonstereotypic oblique stripe of Mylochromis mola. Complementary ontogenetic data give insights into the development of stripe patterns as well as vertical bar patterns that both develop postembryonically. Lastly, using the Lake Victoria species pair Haplochromis sauvagei and Pundamilia nyererei, we investigated the differences between melanic and non-melanic regions to identify additional genes that contribute to the formation of stripes. Expression differences-that most importantly also do not include agrp2-are surprisingly small. This suggests, at least in this species pair, that the stripe phenotype might be caused by a combination of more subtle transcriptomic differences or cellular changes without transcriptional correlates. In summary, our comprehensive analysis highlights the ontogenetic and adult transcriptomic differences between cichlids with different color patterns and serves as a basis for further investigation of the mechanistic underpinnings of their diversification.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

agouti gene family, agrp2, asip2b, Cichlidae, coloration, ontogeny, pigmentation, teleosts

Zitieren

ISO 690

KRATOCHWIL, Claudius F., Yipeng LIANG, Jan GERWIN, Paolo FRANCHINI, Axel MEYER, 2022. Comparative ontogenetic and transcriptomic analyses shed light on color pattern divergence in cichlid fishes. In: Evolution & Development. Wiley-Blackwell - STM. 2022, 24(5), pp. 158-170. ISSN 1520-541X. eISSN 1525-142X. Available under: doi: 10.1111/ede.12416

BibTex

@article{Kratochwil2022-09Compa-58403,
  year={2022},
  doi={10.1111/ede.12416},
  title={Comparative ontogenetic and transcriptomic analyses shed light on color pattern divergence in cichlid fishes},
  number={5},
  volume={24},
  issn={1520-541X},
  journal={Evolution & Development},
  pages={158--170},
  author={Kratochwil, Claudius F. and Liang, Yipeng and Gerwin, Jan and Franchini, Paolo and Meyer, Axel},
  note={Deutsche Forschungsgemeinschaft (DFG) to Axel Meyer, Claudius F. Kratochwil (KR 4670/2-1 and KR 4670/4-1), and Paolo Franchini (FR 3399/1-1)}
}

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Kommentar zur Publikation

Deutsche Forschungsgemeinschaft (DFG) to Axel Meyer, Claudius F. Kratochwil (KR 4670/2-1 and KR 4670/4-1), and Paolo Franchini (FR 3399/1-1)

Universitätsbibliographie

Ja

Begutachtet

Ja