A comprehensive landscape of 60S ribosome biogenesis factors

Sailer, Carolin; Jansen, Jasmin; Sekulski, Kamil; Cruz, Victor E.; Erzberger, Jan P.; Stengel, Florian

A comprehensive landscape of 60S ribosome biogenesis factors

Dateien

Sailer_2-1macxywkv238c5.pdfGröße: 7.5 MBDownloads: 113

Datum

2022

Autor:innen

Sailer, Carolin

Jansen, Jasmin

Sekulski, Kamil

Cruz, Victor E.

Erzberger, Jan P.

Stengel, Florian

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Cell Reports. Cell Press. 2022, 38(6), 110353. eISSN 2211-1247. Available under: doi: 10.1016/j.celrep.2022.110353

Zusammenfassung

Eukaryotic ribosome biogenesis is facilitated and regulated by numerous ribosome biogenesis factors (RBFs). High-resolution cryoelectron microscopy (cryo-EM) maps have defined the molecular interactions of RBFs during maturation, but many transient and dynamic interactions, particularly during early assembly, remain uncharacterized. Using quantitative proteomics and crosslinking coupled to mass spectrometry (XL-MS) data from an extensive set of pre-ribosomal particles, we derive a comprehensive and time-resolved interaction map of RBF engagement during 60S maturation. We localize 22 previously unmapped RBFs to specific biogenesis intermediates and validate our results by mapping the catalytic activity of the methyltransferases Bmt2 and Rcm1 to their predicted nucleolar 60S intermediates. Our analysis reveals the interaction sites for the RBFs Noc2 and Ecm1 and elucidates the interaction map and timing of 60S engagement by the DEAD-box ATPases Dbp9 and Dbp10. Our data provide a powerful resource for future studies of 60S ribosome biogenesis.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

SAILER, Carolin, Jasmin JANSEN, Kamil SEKULSKI, Victor E. CRUZ, Jan P. ERZBERGER, Florian STENGEL, 2022. A comprehensive landscape of 60S ribosome biogenesis factors. In: Cell Reports. Cell Press. 2022, 38(6), 110353. eISSN 2211-1247. Available under: doi: 10.1016/j.celrep.2022.110353

BibTex

@article{Sailer2022compr-56821,
  year={2022},
  doi={10.1016/j.celrep.2022.110353},
  title={A comprehensive landscape of 60S ribosome biogenesis factors},
  number={6},
  volume={38},
  journal={Cell Reports},
  author={Sailer, Carolin and Jansen, Jasmin and Sekulski, Kamil and Cruz, Victor E. and Erzberger, Jan P. and Stengel, Florian},
  note={Article Number: 110353}
}

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