KOPS - The Institutional Repository of the University of Konstanz

Assessing Genotoxicity of Ten Different Engineered Nanomaterials by the Novel Semi-Automated FADU Assay and the Alkaline Comet Assay

Assessing Genotoxicity of Ten Different Engineered Nanomaterials by the Novel Semi-Automated FADU Assay and the Alkaline Comet Assay

Cite This

Files in this item

Checksum: MD5:92f146c106f28feae3a495e40f12d6bd

MAY, Sarah, Cordula HIRSCH, Alexandra RIPPL, Alexander BÜRKLE, Peter WICK, 2022. Assessing Genotoxicity of Ten Different Engineered Nanomaterials by the Novel Semi-Automated FADU Assay and the Alkaline Comet Assay. In: Nanomaterials. MDPI. 12(2), 220. eISSN 2079-4991. Available under: doi: 10.3390/nano12020220

@article{May2022-01-10Asses-56415, title={Assessing Genotoxicity of Ten Different Engineered Nanomaterials by the Novel Semi-Automated FADU Assay and the Alkaline Comet Assay}, year={2022}, doi={10.3390/nano12020220}, number={2}, volume={12}, journal={Nanomaterials}, author={May, Sarah and Hirsch, Cordula and Rippl, Alexandra and Bürkle, Alexander and Wick, Peter}, note={Article Number: 220} }

Attribution 4.0 International 2022-02-02T08:29:48Z Bürkle, Alexander Rippl, Alexandra May, Sarah Wick, Peter Increased engineered nanomaterial (ENM) production and incorporation in consumer and biomedical products has raised concerns about the potential adverse effects. The DNA damaging capacity is of particular importance since damaged genetic material can lead to carcinogenesis. Consequently, reliable and robust in vitro studies assessing ENM genotoxicity are of great value. We utilized two complementary assays based on different measurement principles: (1) comet assay and (2) FADU (fluorimetric detection of alkaline DNA unwinding) assay. Assessing cell viability ruled out false-positive results due to DNA fragmentation during cell death. Potential structure–activity relationships of 10 ENMs were investigated: three silica nanoparticles (SiO<sub>2</sub>-NP) with varying degrees of porosity, titanium dioxide (TiO<sub>2</sub>-NP), polystyrene (PS-NP), zinc oxide (ZnO-NP), gold (Au-NP), graphene oxide (GO) and two multi-walled carbon nanotubes (MWNT). SiO<sub>2</sub>-NPs, TiO<sub>2</sub>-NP and GO were neither cytotoxic nor genotoxic to Jurkat E6-I cells. Quantitative interference corrections derived from GO results can make the FADU assay a promising screening tool for a variety of ENMs. MWNT merely induced cytotoxicity, while dose- and time-dependent cytotoxicity of PS-NP was accompanied by DNA fragmentation. Hence, PS-NP served to benchmark threshold levels of cytotoxicity at which DNA fragmentation was expected. Considering all controls revealed the true genotoxicity for Au-NP and ZnO-NP at early time Points. eng Hirsch, Cordula Hirsch, Cordula 2022-01-10 2022-02-02T08:29:48Z Bürkle, Alexander Rippl, Alexandra Assessing Genotoxicity of Ten Different Engineered Nanomaterials by the Novel Semi-Automated FADU Assay and the Alkaline Comet Assay May, Sarah Wick, Peter

Downloads since Feb 2, 2022 (Information about access statistics)

May_2-17x4g3nqmvptc1.pdf 55

This item appears in the following Collection(s)

Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International

Search KOPS


Browse

My Account