Optogenetic investigation of BMP target gene expression diversity

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ROGERS, Katherine W., Mohammad ELGAMACY, Benjamin M. JORDAN, Patrick MÜLLER, 2020. Optogenetic investigation of BMP target gene expression diversity. In: eLife. eLife Sciences Publications. 9, e58641. eISSN 2050-084X. Available under: doi: 10.7554/eLife.58641

@article{Rogers2020Optog-56053, title={Optogenetic investigation of BMP target gene expression diversity}, year={2020}, doi={10.7554/eLife.58641}, volume={9}, journal={eLife}, author={Rogers, Katherine W. and ElGamacy, Mohammad and Jordan, Benjamin M. and Müller, Patrick}, note={Article Number: e58641} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/56053"> <dc:creator>ElGamacy, Mohammad</dc:creator> <dc:creator>Rogers, Katherine W.</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-01-04T09:34:32Z</dcterms:available> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/56053/3/Rogers_2-15pffs7vw4myd8.pdf"/> <dc:contributor>Jordan, Benjamin M.</dc:contributor> <dc:creator>Müller, Patrick</dc:creator> <dc:contributor>ElGamacy, Mohammad</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/56053"/> <dcterms:abstract xml:lang="eng">Signaling molecules activate distinct patterns of gene expression to coordinate embryogenesis, but how spatiotemporal expression diversity is generated is an open question. In zebrafish, a BMP signaling gradient patterns the dorsal-ventral axis. We systematically identified target genes responding to BMP and found that they have diverse spatiotemporal expression patterns. Transcriptional responses to optogenetically delivered high- and low-amplitude BMP signaling pulses indicate that spatiotemporal expression is not fully defined by different BMP signaling activation thresholds. Additionally, we observed negligible correlations between spatiotemporal expression and transcription kinetics for the majority of analyzed genes in response to BMP signaling pulses. In contrast, spatial differences between BMP target genes largely collapsed when FGF and Nodal signaling were inhibited. Our results suggest that, similar to other patterning systems, combinatorial signaling is likely to be a major driver of spatial diversity in BMP-dependent gene expression in zebrafish.</dcterms:abstract> <dc:rights>Attribution 4.0 International</dc:rights> <dc:contributor>Rogers, Katherine W.</dc:contributor> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/56053/3/Rogers_2-15pffs7vw4myd8.pdf"/> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <dcterms:title>Optogenetic investigation of BMP target gene expression diversity</dcterms:title> <dc:contributor>Müller, Patrick</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:issued>2020</dcterms:issued> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-01-04T09:34:32Z</dc:date> <dc:creator>Jordan, Benjamin M.</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>

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