KOPS - The Institutional Repository of the University of Konstanz

In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code

In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code

Cite This

Files in this item

Checksum: MD5:aa1f8c25fc6884509d90bbf0e547ca2a

KUGELE, Anandi, Sophie KETTER, Bjarne SILKENATH, Valentin WITTMANN, Benesh JOSEPH, Malte DRESCHER, 2021. In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code. In: Chemical Communications. Royal Society of Chemistry (RSC). 57(96), pp. 12980-12983. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/d1cc04612h

@article{Kugele2021-11-18spect-55826, title={In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code}, year={2021}, doi={10.1039/d1cc04612h}, number={96}, volume={57}, issn={1359-7345}, journal={Chemical Communications}, pages={12980--12983}, author={Kugele, Anandi and Ketter, Sophie and Silkenath, Bjarne and Wittmann, Valentin and Joseph, Benesh and Drescher, Malte} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/55826"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-12-09T13:02:08Z</dc:date> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/3.0/"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/55826"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:abstract xml:lang="eng">The membrane transporter BtuB is site-directedly spin labelled on the surface of living Escherichia coli via Diels–Alder click chemistry of the genetically encoded amino acid SCO-L-lysine. The previously introduced photoactivatable nitroxide PaNDA prevents off-target labelling, is used for distance measurements, and the temporally shifted activation of the nitroxide allows for advanced experimental setups. This study describes significant evolution of Diels–Alder-mediated spin labelling on cellular surfaces and opens up new vistas for the the study of membrane proteins.</dcterms:abstract> <dcterms:issued>2021-11-18</dcterms:issued> <dc:creator>Wittmann, Valentin</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:contributor>Silkenath, Bjarne</dc:contributor> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/55826/1/Kugele_2-40i8514f6nr3.pdf"/> <dc:contributor>Joseph, Benesh</dc:contributor> <dc:creator>Kugele, Anandi</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:creator>Ketter, Sophie</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Wittmann, Valentin</dc:contributor> <dc:contributor>Kugele, Anandi</dc:contributor> <dc:language>eng</dc:language> <dcterms:title>In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code</dcterms:title> <dc:creator>Joseph, Benesh</dc:creator> <dc:contributor>Ketter, Sophie</dc:contributor> <dc:creator>Silkenath, Bjarne</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/55826/1/Kugele_2-40i8514f6nr3.pdf"/> <dc:creator>Drescher, Malte</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-12-09T13:02:08Z</dcterms:available> <dc:rights>Attribution 3.0 Unported</dc:rights> <dc:contributor>Drescher, Malte</dc:contributor> </rdf:Description> </rdf:RDF>

Downloads since Dec 9, 2021 (Information about access statistics)

Kugele_2-40i8514f6nr3.pdf 50

This item appears in the following Collection(s)

Attribution 3.0 Unported Except where otherwise noted, this item's license is described as Attribution 3.0 Unported

Search KOPS


Browse

My Account