KOPS - The Institutional Repository of the University of Konstanz

Cytosolic proline is required for basal freezing tolerance in Arabidopsis

Cytosolic proline is required for basal freezing tolerance in Arabidopsis

Cite This

Files in this item

Checksum: MD5:415bd5ec162b40c373ac4cdc9a9eca7e

HOERMILLER, Imke I., Dietmar FUNCK, Lilli SCHÖNEWOLF, Henrik MAY, Arnd G. HEYER, 2022. Cytosolic proline is required for basal freezing tolerance in Arabidopsis. In: Plant, Cell & Environment. Wiley. 45(1), pp. 147-155. ISSN 0140-7791. eISSN 1365-3040. Available under: doi: 10.1111/pce.14196

@article{Hoermiller2022-01Cytos-55407, title={Cytosolic proline is required for basal freezing tolerance in Arabidopsis}, year={2022}, doi={10.1111/pce.14196}, number={1}, volume={45}, issn={0140-7791}, journal={Plant, Cell & Environment}, pages={147--155}, author={Hoermiller, Imke I. and Funck, Dietmar and Schönewolf, Lilli and May, Henrik and Heyer, Arnd G.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/55407"> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Schönewolf, Lilli</dc:creator> <dc:creator>Hoermiller, Imke I.</dc:creator> <dc:creator>May, Henrik</dc:creator> <dc:contributor>Hoermiller, Imke I.</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/55407/1/Hoermiller_2-1q6c930w98yli5.pdf"/> <dc:contributor>May, Henrik</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-10-29T07:05:01Z</dc:date> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/55407/1/Hoermiller_2-1q6c930w98yli5.pdf"/> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/4.0/"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:rights>Attribution-NonCommercial-NoDerivatives 4.0 International</dc:rights> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Funck, Dietmar</dc:contributor> <dcterms:title>Cytosolic proline is required for basal freezing tolerance in Arabidopsis</dcterms:title> <dc:creator>Funck, Dietmar</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/55407"/> <dc:contributor>Heyer, Arnd G.</dc:contributor> <dcterms:abstract xml:lang="eng">The amino acid proline accumulates in many plant species under abiotic stress conditions, and various protective functions have been proposed. During cold stress, however, proline content in Arabidopsis thaliana does not correlate with freezing tolerance. Freezing sensitivity of a starchless plastidic phosphoglucomutase mutant (pgm) indicated that localization of proline in the cytosol might stabilize the plasma membrane during freeze–thaw events. Here, we show that re-allocation of proline from cytosol to vacuole was similar in the pyrroline-5-carboxylate synthase 2–1 (p5cs2–1) mutant and the pgm mutant and caused similar reduction of basal freezing tolerance. In contrast, the starch excess 1–1 mutant (sex1-1) had even lower freezing tolerance than pgm but did not affect sub-cellular localization of proline. Freezing sensitivity of sex1-1 mutants affected primarily the photosynthetic electron transport and was enhanced in a sex1-1::p5cs2–1 double mutant. These findings indicate that several independent factors determine basal freezing tolerance. In a pgm::p5cs2–1 double mutant, freezing sensitivity and proline allocation to the vacuole were the same as in the parental lines, indicating that the lack of cytosolic proline was the common cause of reduced basal freezing tolerance in both mutants. We conclude that cytosolic proline is an important factor in freezing tolerance of non-acclimated plants.</dcterms:abstract> <dcterms:issued>2022-01</dcterms:issued> <dc:contributor>Schönewolf, Lilli</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:language>eng</dc:language> <dc:creator>Heyer, Arnd G.</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-10-29T07:05:01Z</dcterms:available> </rdf:Description> </rdf:RDF>

Downloads since Oct 29, 2021 (Information about access statistics)

Hoermiller_2-1q6c930w98yli5.pdf 46

This item appears in the following Collection(s)

Attribution-NonCommercial-NoDerivatives 4.0 International Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International

Search KOPS


Browse

My Account