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Development of a novel method for the identification and differentiation of micro- and nanoplastics based on time resolved fluorescence processes

Development of a novel method for the identification and differentiation of micro- and nanoplastics based on time resolved fluorescence processes

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MONTELEONE, Adrian, 2021. Development of a novel method for the identification and differentiation of micro- and nanoplastics based on time resolved fluorescence processes [Dissertation]. Konstanz: University of Konstanz

@phdthesis{Monteleone2021Devel-54864, title={Development of a novel method for the identification and differentiation of micro- and nanoplastics based on time resolved fluorescence processes}, year={2021}, author={Monteleone, Adrian}, address={Konstanz}, school={Universität Konstanz} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/54864"> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/54864/3/Monteleone_2-1xtejs8gkw9ri5.pdf"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Monteleone, Adrian</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-09-13T09:37:34Z</dcterms:available> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Monteleone, Adrian</dc:creator> <dcterms:issued>2021</dcterms:issued> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-09-13T09:37:34Z</dc:date> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/54864/3/Monteleone_2-1xtejs8gkw9ri5.pdf"/> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:rights>terms-of-use</dc:rights> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:language>eng</dc:language> <dcterms:abstract xml:lang="eng">In summary, the results of this dissertation show that autofluorescence is a remarkable property of plastics and that it experiences an increase in intensity when incubation is performed at high temperatures. This increased intensity of fluorescence is only partially sufficient for modern methodology and is more suitable for detection or contamination prediction. The integration of the temporal resolution of these fluorescence processes allows an extension to perform a specific characterization, as well as differentiation of the micro- and nanoplastic. The combination of mean intensity-weighted τ and mean amplitude-weighted τ values allowed, under the given settings, the significant differentiation of 94.55 % of the included plastics with and without elevated temperature treatment. Phasor analysis speeds up the measurement, allows analysis of a larger amount of image data (high throughput), and reduces from bias by avoiding fitting algorithms and enhanced the analysis with helpful interactive tools. Applicability in more complex matrices or in deeper tissues could not be realized at this stage and needs follow-up experiments. Furthermore, FLIM in combination with phasor analysis showed the potential for submicron analysis of plastics, 3D sections of tissue and environmental samples. Furthermore, technologies such as multiphoton FLIM, STED-FLIM and new algorithms for image analysis are available for further experiments and developments.</dcterms:abstract> <dcterms:title>Development of a novel method for the identification and differentiation of micro- and nanoplastics based on time resolved fluorescence processes</dcterms:title> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/54864"/> </rdf:Description> </rdf:RDF>

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