KOPS - The Institutional Repository of the University of Konstanz

Deciphering molecular details of the RAC-ribosome interaction by EPR spectroscopy

Deciphering molecular details of the RAC-ribosome interaction by EPR spectroscopy

Cite This

Files in this item

Checksum: MD5:8690a3d3e078f240ff9d71d8a6ae4077

FRIES, Sandra J., Theresa S. BRAUN, Christoph GLOBISCH, Christine PETER, Malte DRESCHER, Elke DEUERLING, 2021. Deciphering molecular details of the RAC-ribosome interaction by EPR spectroscopy. In: Scientific Reports. Springer. 11(1), 8681. eISSN 2045-2322. Available under: doi: 10.1038/s41598-021-87847-y

@article{Fries2021-04-21Decip-53495, title={Deciphering molecular details of the RAC-ribosome interaction by EPR spectroscopy}, year={2021}, doi={10.1038/s41598-021-87847-y}, number={1}, volume={11}, journal={Scientific Reports}, author={Fries, Sandra J. and Braun, Theresa S. and Globisch, Christoph and Peter, Christine and Drescher, Malte and Deuerling, Elke}, note={Article Number: 8681} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/53495"> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Globisch, Christoph</dc:contributor> <dc:contributor>Drescher, Malte</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-04-27T07:21:57Z</dc:date> <dc:creator>Globisch, Christoph</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dcterms:issued>2021-04-21</dcterms:issued> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-04-27T07:21:57Z</dcterms:available> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Drescher, Malte</dc:creator> <dcterms:title>Deciphering molecular details of the RAC-ribosome interaction by EPR spectroscopy</dcterms:title> <dc:rights>Attribution 4.0 International</dc:rights> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Braun, Theresa S.</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/53495/1/Fries_2-y4rprkb6p5219.pdf"/> <dc:creator>Peter, Christine</dc:creator> <dc:contributor>Peter, Christine</dc:contributor> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <dc:language>eng</dc:language> <dc:creator>Deuerling, Elke</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/53495/1/Fries_2-y4rprkb6p5219.pdf"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/53495"/> <dc:creator>Fries, Sandra J.</dc:creator> <dc:creator>Braun, Theresa S.</dc:creator> <dcterms:abstract xml:lang="eng">The eukaryotic ribosome-associated complex (RAC) plays a significant role in de novo protein folding. Its unique interaction with the ribosome, comprising contacts to both ribosomal subunits, suggests a RAC-mediated coordination between translation elongation and co-translational protein folding. Here, we apply electron paramagnetic resonance (EPR) spectroscopy combined with site-directed spin labeling (SDSL) to gain deeper insights into a RAC-ribosome contact affecting translational accuracy. We identified a local contact point of RAC to the ribosome. The data provide the first experimental evidence for the existence of a four-helix bundle as well as a long α-helix in full-length RAC, in solution as well as on the ribosome. Additionally, we complemented the structural picture of the region mediating this functionally important contact on the 40S ribosomal subunit. In sum, this study constitutes the first application of SDSL-EPR spectroscopy to elucidate the molecular details of the interaction between the 3.3 MDa translation machinery and a chaperone complex.</dcterms:abstract> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:contributor>Deuerling, Elke</dc:contributor> <dc:contributor>Fries, Sandra J.</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> </rdf:Description> </rdf:RDF>

Downloads since Apr 27, 2021 (Information about access statistics)

Fries_2-y4rprkb6p5219.pdf 29

This item appears in the following Collection(s)

Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International

Search KOPS


Browse

My Account