KOPS - The Institutional Repository of the University of Konstanz

Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood

Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood

Cite This

Files in this item

Checksum: MD5:797077873455b4bcfb2aed59ae9dba10

MINOPOLI, Antonio, Bartolomeo DELLA VENTURA, Bohdan LENYK, Francesco GENTILE, Julian A. TANNER, Andreas OFFENHÄUSSER, Dirk MAYER, Raffaele VELOTTA, 2020. Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood. In: Nature communications. Nature Publishing Group. 11(1), 6134. eISSN 2041-1723. Available under: doi: 10.1038/s41467-020-19755-0

@article{Minopoli2020Ultra-52292, title={Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood}, year={2020}, doi={10.1038/s41467-020-19755-0}, number={1}, volume={11}, journal={Nature communications}, author={Minopoli, Antonio and Della Ventura, Bartolomeo and Lenyk, Bohdan and Gentile, Francesco and Tanner, Julian A. and Offenhäusser, Andreas and Mayer, Dirk and Velotta, Raffaele}, note={Article Number: 6134} }

Lenyk, Bohdan Attribution 4.0 International Gentile, Francesco Development of plasmonic biosensors combining reliability and ease of use is still a challenge. Gold nanoparticle arrays made by block copolymer micelle nanolithography (BCMN) stand out for their scalability, cost-effectiveness and tunable plasmonic properties, making them ideal substrates for fluorescence enhancement. Here, we describe a plasmon-enhanced fluorescence immunosensor for the specific and ultrasensitive detection of Plasmodium falciparum lactate dehydrogenase (PfLDH)-a malaria marker-in whole blood. Analyte recognition is realized by oriented antibodies immobilized in a close-packed configuration via the photochemical immobilization technique (PIT), with a top bioreceptor of nucleic acid aptamers recognizing a different surface of PfLDH in a sandwich conformation. The combination of BCMN and PIT enabled maximum control over the nanoparticle size and lattice constant as well as the distance of the fluorophore from the sensing surface. The device achieved a limit of detection smaller than 1 pg/mL (<30 fM) with very high specificity without any sample pretreatment. This limit of detection is several orders of magnitude lower than that found in malaria rapid diagnostic tests or even commercial ELISA kits. Thanks to its overall dimensions, ease of use and high-throughput analysis, the device can be used as a substrate in automated multi-well plate readers and improve the efficiency of conventional fluorescence immunoassays. Lenyk, Bohdan Gentile, Francesco Mayer, Dirk Della Ventura, Bartolomeo Tanner, Julian A. Velotta, Raffaele Minopoli, Antonio 2020 Mayer, Dirk Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood Offenhäusser, Andreas Minopoli, Antonio Velotta, Raffaele Tanner, Julian A. eng 2021-01-05T12:57:43Z Offenhäusser, Andreas 2021-01-05T12:57:43Z Della Ventura, Bartolomeo

Downloads since Jan 5, 2021 (Information about access statistics)

Minopoli_2-dvnh9d22ov1a1.pdf 44

This item appears in the following Collection(s)

Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International

Search KOPS


Browse

My Account