A ligand selection strategy identifies chemical probes targeting the proteases of SARS‐CoV‐2

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Angewandte Chemie International Edition. Wiley. 2021, 60(12), pp. 6799-6806. ISSN 1433-7851. eISSN 1521-3773. Available under: doi: 10.1002/anie.202016113
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Activity‐based probes are valuable tools for chemical biology. However, finding probes that specifically target the active site of an enzyme remains a challenging task. Here we present a ligand selection strategy that allows to rapidly tailor electrophilic probes to a target of choice and showcase its application for the two cysteine proteases of SARS‐CoV‐2 as proof of concept. The resulting probes were specific for the active site labelling of 3CL pro and PL pro with sufficient selectivity in a live cell model as well as in the background of a native human proteome. Exploiting the probes as tools for competitive profiling of a natural product library identified salvianolic acid derivatives as promising 3CL pro inhibitors. We anticipate that our ligand selection strategy will be useful to rapidly develop customized probes and discover inhibitors for a wide range of target proteins also beyond corona virus proteases.

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ISO 690PENALVER, Lilian, Philipp SCHMID, David SZAMOSVARI, Stefan SCHILDKNECHT, Christoph GLOBISCH, Kevin SAWADE, Christine PETER, Thomas BÖTTCHER, 2021. A ligand selection strategy identifies chemical probes targeting the proteases of SARS‐CoV‐2. In: Angewandte Chemie International Edition. Wiley. 2021, 60(12), pp. 6799-6806. ISSN 1433-7851. eISSN 1521-3773. Available under: doi: 10.1002/anie.202016113
BibTex
@article{Penalver2021-03-15ligan-52228,
  year={2021},
  doi={10.1002/anie.202016113},
  title={A ligand selection strategy identifies chemical probes targeting the proteases of SARS‐CoV‐2},
  number={12},
  volume={60},
  issn={1433-7851},
  journal={Angewandte Chemie International Edition},
  pages={6799--6806},
  author={Penalver, Lilian and Schmid, Philipp and Szamosvari, David and Schildknecht, Stefan and Globisch, Christoph and Sawade, Kevin and Peter, Christine and Böttcher, Thomas}
}
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