KOPS - The Institutional Repository of the University of Konstanz

Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide

Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide

Cite This

Files in this item

Checksum: MD5:236ff18ea13881c4db368e22027d6356

WELTER, Moritz, Andreas MARX, 2020. Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide. In: Chemistry. MDPI. 2(2), pp. 490-498. eISSN 2624-8549. Available under: doi: 10.3390/chemistry2020029

@article{Welter2020-06Combi-52032, title={Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide}, year={2020}, doi={10.3390/chemistry2020029}, number={2}, volume={2}, journal={Chemistry}, pages={490--498}, author={Welter, Moritz and Marx, Andreas} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/52032"> <dcterms:title>Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide</dcterms:title> <dc:language>eng</dc:language> <dc:creator>Welter, Moritz</dc:creator> <dc:rights>Attribution 4.0 International</dc:rights> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/52032/3/Welter_2-pl43enl37msc9.pdf"/> <dcterms:issued>2020-06</dcterms:issued> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:contributor>Welter, Moritz</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-12-07T13:33:18Z</dcterms:available> <dcterms:abstract xml:lang="eng">LAMP is an approach for isothermal nucleic acids diagnostics with increasing importance but suffers from the need of tedious systems design and optimization for every new target. Here, we describe an approach for its simplification based on a single nucleoside-5′-O-triphosphate (dNTP) that is covalently modified with a DNA strand. We found that the DNA-modified dNTP is a substrate for DNA polymerases in versatile primer extension reactions despite its size and that the incorporated DNA indeed serves as a target for selective LAMP analysis.</dcterms:abstract> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/52032/3/Welter_2-pl43enl37msc9.pdf"/> <dc:contributor>Marx, Andreas</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-12-07T13:33:18Z</dc:date> <dc:creator>Marx, Andreas</dc:creator> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/52032"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> </rdf:Description> </rdf:RDF>

Downloads since Dec 7, 2020 (Information about access statistics)

Welter_2-pl43enl37msc9.pdf 100

This item appears in the following Collection(s)

Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International

Search KOPS


Browse

My Account