KOPS - The Institutional Repository of the University of Konstanz

Chemical concentrations in cell culture compartments (C5) : free concentrations

Chemical concentrations in cell culture compartments (C5) : free concentrations

Cite This

Files in this item

Checksum: MD5:a1c3f50d99fac627d93827d3a4825d09

KISITU, Jaffar, Henner HOLLERT, Ciarán FISHER, Marcel LEIST, 2020. Chemical concentrations in cell culture compartments (C5) : free concentrations. In: Alternatives to Animal Experimentation : ALTEX. Springer Spektrum. 37(4), pp. 693-708. ISSN 1868-596X. eISSN 1868-8551. Available under: doi: 10.14573/altex.2008251

@article{Kisitu2020Chemi-51796, title={Chemical concentrations in cell culture compartments (C5) : free concentrations}, year={2020}, doi={10.14573/altex.2008251}, number={4}, volume={37}, issn={1868-596X}, journal={Alternatives to Animal Experimentation : ALTEX}, pages={693--708}, author={Kisitu, Jaffar and Hollert, Henner and Fisher, Ciarán and Leist, Marcel} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/51796"> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/51796"/> <dc:creator>Kisitu, Jaffar</dc:creator> <dc:contributor>Hollert, Henner</dc:contributor> <dcterms:title>Chemical concentrations in cell culture compartments (C5) : free concentrations</dcterms:title> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Fisher, Ciarán</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Fisher, Ciarán</dc:contributor> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <dc:creator>Hollert, Henner</dc:creator> <dcterms:abstract xml:lang="eng">In biological systems (cell culture media, cells, body fluids), drugs/toxicants are usually not freely dissolved but partially bound to biomolecules; only a fraction of the chemical is free/unbound (fu). To predict pharmacological effects and toxicity, it is important that the fu of the drug is known. As the differences between free and nominal concentrations are determined by test system parameters (e.g., the protein and lipid content, and the type of surface material), comparison of nominal concentrations between two different new approach methods (NAM) may lead to faulty conclusions. The same problem exists when in vitro concentrations are compared to those in human subjects. Therefore, the respective fu of a chemical in a test system needs to be determined for in vitro-to-in vivo extrapolations (IVIVE). Besides direct measurements, prediction models can help to obtain fu. Here we describe a simplified approach to approximate fu and provide background information on the underlying assumptions. Comparative predictions and measurements of fu of various drugs are shown to exemplify the approach. Basic input data, like protein and lipid concentrations, are also provided. Beyond such test systems data, the only required chemical-specific inputs are the lipophilicity of the candidate drug and its ionization state, as determined by the dissociation constants of its acidic or basic groups. This overview is intended to be used by any lab scientist without specific toxicokinetics training to obtain an estimate of fu in a given cell culture medium.</dcterms:abstract> <dc:contributor>Kisitu, Jaffar</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Leist, Marcel</dc:contributor> <dc:creator>Leist, Marcel</dc:creator> <dcterms:issued>2020</dcterms:issued> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-11-16T11:53:37Z</dc:date> <dc:rights>Attribution 4.0 International</dc:rights> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/51796/1/Kisitu_2-1t853tp955kuk7.pdf"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-11-16T11:53:37Z</dcterms:available> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/51796/1/Kisitu_2-1t853tp955kuk7.pdf"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> </rdf:Description> </rdf:RDF>

Downloads since Nov 16, 2020 (Information about access statistics)

Kisitu_2-1t853tp955kuk7.pdf 97

This item appears in the following Collection(s)

Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International

Search KOPS


Browse

My Account