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Coherent anti-Stokes Raman scattering microspectroscopy based on a compact Er:fiber laser

Coherent anti-Stokes Raman scattering microspectroscopy based on a compact Er:fiber laser

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SELM, Romedi, Martin WINTERHALDER, Andrea NAGY, Andreas ZUMBUSCH, Günther KRAUSS, Tobias HANKE, Alexander SELL, Alfred LEITENSTORFER, 2011. Coherent anti-Stokes Raman scattering microspectroscopy based on a compact Er:fiber laser. SPIE BIOS. San Francisco, California, USA, Jan 23, 2011 - Jan 25, 2011. In: PERIASAMY, Ammasi, ed. and others. Multiphoton microscopy in the biomedical sciences XI : 23 - 25 January 2011, San Francisco, California, United States ; [part of SPIE photonics west, the SPIE BIOS 2011 meeting]. Bellingham, Wash.:SPIE, 79030Y. eISSN 0277-786X. ISBN 978-0-8194-8440-6. Available under: doi: 10.1117/12.874938

@inproceedings{Selm2011-02-10Coher-51209, title={Coherent anti-Stokes Raman scattering microspectroscopy based on a compact Er:fiber laser}, year={2011}, doi={10.1117/12.874938}, number={7903}, isbn={978-0-8194-8440-6}, address={Bellingham, Wash.}, publisher={SPIE}, series={Proceedings of SPIE}, booktitle={Multiphoton microscopy in the biomedical sciences XI : 23 - 25 January 2011, San Francisco, California, United States ; [part of SPIE photonics west, the SPIE BIOS 2011 meeting]}, editor={Periasamy, Ammasi}, author={Selm, Romedi and Winterhalder, Martin and Nagy, Andrea and Zumbusch, Andreas and Krauss, Günther and Hanke, Tobias and Sell, Alexander and Leitenstorfer, Alfred}, note={Article Number: 79030Y} }

Coherent anti-Stokes Raman scattering microspectroscopy based on a compact Er:fiber laser Winterhalder, Martin Zumbusch, Andreas eng Krauss, Günther Selm, Romedi Leitenstorfer, Alfred Leitenstorfer, Alfred 2020-10-06T07:27:21Z Sell, Alexander Nagy, Andrea Selm, Romedi Zumbusch, Andreas Nagy, Andrea A two branch Er:fiber laser was developed for coherent anti-Stokes Raman scattering (CARS) microspectroscopy. The compact and highly stable light source allows for fast single-frequency CARS microspectroscopy with a wide tuning range from 1150 cm<sup>-1</sup> up to 3800 cm<sup>-1</sup>. Single-pass frequency conversion enables easy tunability. The spectral selectivity of the system is shown using polymer beads. Imaging of biological samples is demonstrated on C. elegans and yeast cells. Modification of the light source allows for broadband background-free CARS microspectroscopy. Impulsive excitation of molecular resonances is achieved using an 11 fs pulse at 1210 nm. Broadband excitation gives access to molecular resonances from 0 cm<sup>-1</sup> up to 4000 cm<sup>-1</sup>. Time-delayed narrowband probing at 775 nm enables sensitive and high-speed spectral detection of the CARS signal, free of nonresonant background with a resolution of 10 cm<sup>-1</sup>. 2020-10-06T07:27:21Z Hanke, Tobias Winterhalder, Martin Hanke, Tobias 2011-02-10 Sell, Alexander Krauss, Günther

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