KOPS - The Institutional Repository of the University of Konstanz

In situ microscopic cytometry enables noninvasive viability assessment of animal cells by measuring entropy states

In situ microscopic cytometry enables noninvasive viability assessment of animal cells by measuring entropy states

Cite This

Files in this item

Files Size Format View

There are no files associated with this item.

WIEDEMANN, Philipp, Jean S. GUEZ, Hans B. WIEGEMANN, Florian EGNER, Juan C. QUINTANA, Diego ASANZA-MALDONADO, Marcos FILIPAKI, Jeff WILKESMAN, Christian SCHWIEBERT, Jean P. CASSAR, 2011. In situ microscopic cytometry enables noninvasive viability assessment of animal cells by measuring entropy states. In: Biotechnology and Bioengineering. Wiley-Blackwell. 108(12), pp. 2884-2893. ISSN 0368-1467. eISSN 1097-0290. Available under: doi: 10.1002/bit.23252

@article{Wiedemann2011-12micro-51091, title={In situ microscopic cytometry enables noninvasive viability assessment of animal cells by measuring entropy states}, year={2011}, doi={10.1002/bit.23252}, number={12}, volume={108}, issn={0368-1467}, journal={Biotechnology and Bioengineering}, pages={2884--2893}, author={Wiedemann, Philipp and Guez, Jean S. and Wiegemann, Hans B. and Egner, Florian and Quintana, Juan C. and Asanza-Maldonado, Diego and Filipaki, Marcos and Wilkesman, Jeff and Schwiebert, Christian and Cassar, Jean P.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/51091"> <dcterms:issued>2011-12</dcterms:issued> <dc:contributor>Wilkesman, Jeff</dc:contributor> <dc:creator>Egner, Florian</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/36"/> <dc:creator>Asanza-Maldonado, Diego</dc:creator> <dc:creator>Wiedemann, Philipp</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/36"/> <dc:contributor>Schwiebert, Christian</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-09-29T09:30:50Z</dcterms:available> <dc:contributor>Filipaki, Marcos</dc:contributor> <dc:contributor>Cassar, Jean P.</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-09-29T09:30:50Z</dc:date> <dc:creator>Schwiebert, Christian</dc:creator> <dc:contributor>Guez, Jean S.</dc:contributor> <dc:creator>Quintana, Juan C.</dc:creator> <dc:contributor>Egner, Florian</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/51091"/> <dc:creator>Cassar, Jean P.</dc:creator> <dc:contributor>Asanza-Maldonado, Diego</dc:contributor> <dc:creator>Wiegemann, Hans B.</dc:creator> <dc:creator>Guez, Jean S.</dc:creator> <dc:contributor>Quintana, Juan C.</dc:contributor> <dc:contributor>Wiedemann, Philipp</dc:contributor> <dc:creator>Filipaki, Marcos</dc:creator> <dcterms:abstract xml:lang="eng">Current state of the art to determine the viability of animal cell suspension cultures is based on sampling and subsequent counting using specific staining assays. We demonstrate for the first time a noninvasive in situ imaging cytometry capable of determining the statistics of a morphologic transition during cell death in suspension cultures. To this end, we measure morphometric inhomogeneity—defined as information entropy—in cell in situ micrographs. We found that the cells are partitioned into two discrete entropy states broadened by phenotypical variability. During the normal course of a culture or by inducing cell death, we observe the transition of cells between these states. As shown by comparison with ex situ diagnostics, the entropy transition happens before or while the cytoplasmatic membrane is loosing its ability to exclude charged dyes. Therefore, measurement of morphometric inhomogeneity constitutes a noninvasive assessment of viability in real time.</dcterms:abstract> <dcterms:title>In situ microscopic cytometry enables noninvasive viability assessment of animal cells by measuring entropy states</dcterms:title> <dc:language>eng</dc:language> <dc:contributor>Wiegemann, Hans B.</dc:contributor> <dc:creator>Wilkesman, Jeff</dc:creator> </rdf:Description> </rdf:RDF>

This item appears in the following Collection(s)

Search KOPS


Browse

My Account