Reversible, meniscus-free molecular combing of long-chain DNA

Cite This

Files in this item

Checksum: MD5:220bfb67e62c35bb428b75b63993f0a5

KOOTA, Juha, Ina SEUFFERT, Roman LEHNER, Jun LI, Thomas GISLER, 2007. Reversible, meniscus-free molecular combing of long-chain DNA. In: Langmuir. 23(18), pp. 9365-9368. ISSN 0743-7463. eISSN 1520-5827. Available under: doi: 10.1021/la7013122

@article{Koota2007Rever-4892, title={Reversible, meniscus-free molecular combing of long-chain DNA}, year={2007}, doi={10.1021/la7013122}, number={18}, volume={23}, issn={0743-7463}, journal={Langmuir}, pages={9365--9368}, author={Koota, Juha and Seuffert, Ina and Lehner, Roman and Li, Jun and Gisler, Thomas} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/4892"> <dc:format>application/pdf</dc:format> <dc:creator>Li, Jun</dc:creator> <dc:creator>Lehner, Roman</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T14:51:07Z</dc:date> <dcterms:issued>2007</dcterms:issued> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/41"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/41"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/4892/1/Reversible_meniscus_free_molecular_combing_of_long_chain_DNA.pdf"/> <dc:creator>Gisler, Thomas</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Li, Jun</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Koota, Juha</dc:creator> <dc:contributor>Lehner, Roman</dc:contributor> <dcterms:abstract xml:lang="eng">We introduce a method for reversibly orienting long-chain DNA on solid hydrophilic substrates without a fluid meniscus. End-tethered ì-DNA mushrooms are elongated by a hydrodynamic flow in the presence of trivalent cations, resulting in electrostatic adsorption of the extended DNA to the surface. By complexation of the cations the part of the DNA which is unspecifically bound to the surface desorbs quantitatively, and the mushroom conformation is restored. With the use of multiple deposition-combing steps, combined with a final desorption step, tethering densities higher than attainable with single deposition steps can be obtained.</dcterms:abstract> <dc:contributor>Gisler, Thomas</dc:contributor> <dc:contributor>Koota, Juha</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Seuffert, Ina</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/52"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/4892/1/Reversible_meniscus_free_molecular_combing_of_long_chain_DNA.pdf"/> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:title>Reversible, meniscus-free molecular combing of long-chain DNA</dcterms:title> <dcterms:bibliographicCitation>First publ. in: Langmuir ; 23 (2007). - S. 9365-9368</dcterms:bibliographicCitation> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/4892"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/52"/> <dc:creator>Seuffert, Ina</dc:creator> <dc:rights>terms-of-use</dc:rights> </rdf:Description> </rdf:RDF>

Downloads since Oct 1, 2014 (Information about access statistics)

Reversible_meniscus_free_molecular_combing_of_long_chain_DNA.pdf 219

This item appears in the following Collection(s)

terms-of-use Except where otherwise noted, this item's license is described as terms-of-use

Search KOPS


Browse

My Account