Restricted until: | Nov 27, 2023 |
Type of Publication: | Dissertation |
Publication status: | Published |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-2-1un125ltfxwbu2 |
Author: | Schober, Alexander Fabian |
Year of publication: | 2019 |
Summary: |
Diatoms are a large group of unicellular microalgae that derived from secondary endocytobiosis, and consequently operate photosynthesis and carbon fixation in the context of a peculiar metabolism. Their mitochondria accommodate unique remnants of their evolutionary past, such as a partial mitochondrial glycolysis or a bacterial Entner-Doudoroff pathway. Their plastids are surrounded by four membranes, and possess pyrenoids that are involved in an elaborate biophysical carbon concentrating mechanism. So far, it is not understood how the first step of the Calvin cycle, the reaction catalyzed by the rubisco, is regulated. However, the concentrations of ATP and NADPH in plastids and mitochondria, which are needed to fix carbon dioxide, where shown to be highly balanced via a tight interconnection between plastidal and mitochondrial metabolism. This work addresses several fundamental aspects of the diatom metabolism in order to gain a better understanding of diatoms photosynthesis and its regulation, with a major focus on carbon acquisition. Firstly, we established two methods for the independent purification of plastid and mitochondria fractions from the diatom Thalassiosira pseudonana. By a combination of physiological and molecular techniques, we demonstrate that the obtained organelles remain structurally intact, performing oxygenic photosynthesis and mitochondrial respiration, respectively. A bioinformatic pipeline was established in order to identify organellar proteins experimentally for mapping metabolic pathways, assessing light harvesting pigments, reassigning ‘GreenCut proteins’ and for testing existing targeting prediction programs.
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Examination date (for dissertations): | Sep 26, 2019 |
Dissertation note: | Doctoral dissertation, University of Konstanz |
Subject (DDC): | 570 Biosciences, Biology |
Keywords: | Diatom Organelles, Isolation, Complex Plastid Fractionation, Proteomics, Rubisco Activase, Calvin Cycle Regulation, Cbbx, AAA+ |
Bibliography of Konstanz: | Yes |
SCHOBER, Alexander Fabian, 2019. Characterization of Complex Organelles and the Dissection of a Pyrenoidal Red-Type Rubisco Activation System of Diatoms [Dissertation]. Konstanz: University of Konstanz
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