KOPS - The Institutional Repository of the University of Konstanz

Enhanced sensitivity to local dynamics in peptides by use of temperature-jump IR-spectroscopy and isotope labeling

Enhanced sensitivity to local dynamics in peptides by use of temperature-jump IR-spectroscopy and isotope labeling

Cite This

Files in this item

Checksum: MD5:33b77d0163bb9657a6ee9b7d701af704

SCHEERER, David, Heng CHI, Dan MCELHENY, Timothy A. KEIDERLING, Karin HAUSER, 2020. Enhanced sensitivity to local dynamics in peptides by use of temperature-jump IR-spectroscopy and isotope labeling. In: Chemistry - A European Journal. Wiley. 26(16), pp. 3524-3534. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.201904497

@article{Scheerer2020Enhan-47776, title={Enhanced sensitivity to local dynamics in peptides by use of temperature-jump IR-spectroscopy and isotope labeling}, year={2020}, doi={10.1002/chem.201904497}, number={16}, volume={26}, issn={0947-6539}, journal={Chemistry - A European Journal}, pages={3524--3534}, author={Scheerer, David and Chi, Heng and McElheny, Dan and Keiderling, Timothy A. and Hauser, Karin} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/47776"> <dc:creator>Chi, Heng</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/47776/1/Scheerer_2-1bt3o0z2hq24z4.pdf"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-12-02T14:58:25Z</dcterms:available> <dc:creator>McElheny, Dan</dc:creator> <dc:contributor>Scheerer, David</dc:contributor> <dc:contributor>Keiderling, Timothy A.</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dcterms:abstract xml:lang="eng">Site-specific isotopic labeling of molecules is a widely used approach in IR-spectroscopy to resolve local contributions to vibrational modes. The induced frequency shift of the corresponding IR band depends on the substituted masses, but also on hydrogen bonding and on vibrational coupling. The impact of these different factors was analyzed with a designed three-stranded β-sheet peptide and by use of selected 13C isotope substitutions at multiple positions in the peptide backbone. Single strand labels give rise to isotopically shifted bands at different frequencies depending on the specific sites, demonstrating sensitivity to the local environment. Cross-strand double and triple labeled peptides exhibited two resolved bands, which could be uniquely assigned to specific residues, whose equilibrium IR indicated only weak local-mode coupling. Temperature-jump IR-laser spectroscopy was applied to monitor structural dynamics and revealed an impressive enhancement of the isotope sensitivity to both local positions and coupling between them as compared to equilibrium FTIR. Site-specific relaxation rates were altered upon introduction of additional cross-strand isotopes. Likewise, the rates for the global β-sheet dynamics were affected in a manner dependent on the distinct relaxation behavior of the labeled oscillator. The study demonstrates that isotope labels do not just provide local structural probes, but they rather sense the dynamic complexity of the molecular environment.</dcterms:abstract> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:issued>2020</dcterms:issued> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <dc:contributor>Hauser, Karin</dc:contributor> <dc:creator>Scheerer, David</dc:creator> <dc:rights>Attribution 4.0 International</dc:rights> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-12-02T14:58:25Z</dc:date> <dcterms:title>Enhanced sensitivity to local dynamics in peptides by use of temperature-jump IR-spectroscopy and isotope labeling</dcterms:title> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/47776/1/Scheerer_2-1bt3o0z2hq24z4.pdf"/> <dc:language>eng</dc:language> <dc:creator>Hauser, Karin</dc:creator> <dc:creator>Keiderling, Timothy A.</dc:creator> <dc:contributor>McElheny, Dan</dc:contributor> <dc:contributor>Chi, Heng</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/47776"/> </rdf:Description> </rdf:RDF>

Downloads since Dec 2, 2019 (Information about access statistics)

Scheerer_2-1bt3o0z2hq24z4.pdf 12

This item appears in the following Collection(s)

Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International

Search KOPS


Browse

My Account