In vitro research reproducibility : Keeping up high standards
In vitro research reproducibility : Keeping up high standards
Date
2019
Authors
Hirsch, Cordula
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
DOI (citable link)
International patent number
Link to the license
EU project number
681002
Project
EUToxRisk21
Open Access publication
Collections
Title in another language
Publication type
Journal article
Publication status
Published
Published in
Frontiers in Pharmacology ; 10 (2019). - 1484. - eISSN 1663-9812
Abstract
Concern regarding the reproducibility of observations in life science research has emerged in recent years, particularly in view of unfavorable experiences with preclinical in vivo research. The use of cell-based systems has increasingly replaced in vivo research and the application of in vitro models enjoys an ever-growing popularity. To avoid repeating past mistakes, high standards of reproducibility and reliability must be established and maintained in the field of in vitro biomedical research. Detailed guidance documenting the appropriate handling of cells has been authored but was received with quite disparate perception by different branches in biomedical research. In that regard, we intend to raise awareness of the reproducibility issue among scientists in all branches of contemporary life science research and their individual responsibility in this matter. We have herein compiled a selection of the most susceptible steps of everyday in vitro cell culture routines that have the potential to influence cell quality and recommend practices to minimize the likelihood of poor cell quality impairing reproducibility with modest investment of time and resources.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Cell culture models, reproducibility, Toxicology, Good cell culture practice, New Approach Methods (NAM) 2
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
HIRSCH, Cordula, Stefan SCHILDKNECHT, 2019. In vitro research reproducibility : Keeping up high standards. In: Frontiers in Pharmacology. 10, 1484. eISSN 1663-9812. Available under: doi: 10.3389/fphar.2019.01484BibTex
@article{Hirsch2019vitro-47652, year={2019}, doi={10.3389/fphar.2019.01484}, title={In vitro research reproducibility : Keeping up high standards}, volume={10}, journal={Frontiers in Pharmacology}, author={Hirsch, Cordula and Schildknecht, Stefan}, note={Article Number: 1484} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/47652"> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <dc:language>eng</dc:language> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/47652"/> <dcterms:abstract xml:lang="eng">Concern regarding the reproducibility of observations in life science research has emerged in recent years, particularly in view of unfavorable experiences with preclinical in vivo research. The use of cell-based systems has increasingly replaced in vivo research and the application of in vitro models enjoys an ever-growing popularity. To avoid repeating past mistakes, high standards of reproducibility and reliability must be established and maintained in the field of in vitro biomedical research. Detailed guidance documenting the appropriate handling of cells has been authored but was received with quite disparate perception by different branches in biomedical research. In that regard, we intend to raise awareness of the reproducibility issue among scientists in all branches of contemporary life science research and their individual responsibility in this matter. We have herein compiled a selection of the most susceptible steps of everyday in vitro cell culture routines that have the potential to influence cell quality and recommend practices to minimize the likelihood of poor cell quality impairing reproducibility with modest investment of time and resources.</dcterms:abstract> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/47652/1/Hirsch_2-10e6snhkxwr5q6.pdf"/> <dc:contributor>Schildknecht, Stefan</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-11-27T11:56:50Z</dcterms:available> <dcterms:issued>2019</dcterms:issued> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/47652/1/Hirsch_2-10e6snhkxwr5q6.pdf"/> <dc:creator>Hirsch, Cordula</dc:creator> <dc:rights>Attribution 4.0 International</dc:rights> <dc:creator>Schildknecht, Stefan</dc:creator> <dcterms:title>In vitro research reproducibility : Keeping up high standards</dcterms:title> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-11-27T11:56:50Z</dc:date> <dc:contributor>Hirsch, Cordula</dc:contributor> </rdf:Description> </rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
Yes
Refereed
Yes