The structural basis for processing of unnatural base pairs by DNA polymerases

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2020
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Chemistry - A European Journal. Wiley. 2020, 26(16), pp. 3446-3463. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.201903525
Zusammenfassung

Unnatural base pairs (UBPs) greatly increase the diversity of DNA and RNA which furthers a broad range of molecular biological and biotechnological approaches. Different candidates have been developed whereby alternative hydrogen-bonding patterns and hydrophobic and packing interactions turned out to be the most promising base-pairing concepts to date. Key in many applications is the highly efficient and selective acceptance of artificial base pairs by DNA polymerases that enables amplification of the modified DNA. In this Review we highlight computational as well as experimental studies that were performed to characterize the pairing behavior of UBPs in free duplex DNA or bound to the active site of KlenTaq DNA polymerase. The structural studies on the one hand elucidate how base pairs lacking hydrogen bonds are accepted by these enzymes and on the other hand highlight the influence of one or several consecutive UBPs on the structure of a DNA double helix. Understanding these concepts facilitates optimization of future UBPs for the manifold fields of applications.

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540 Chemie
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DNA, DNA polymerase structures, KlenTaq DNA, polymerase, nucleobases, unnatural base pairs (UBPs)
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ISO 690MARX, Andreas, Karin BETZ, 2020. The structural basis for processing of unnatural base pairs by DNA polymerases. In: Chemistry - A European Journal. Wiley. 2020, 26(16), pp. 3446-3463. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.201903525
BibTex
@article{Marx2020struc-47017,
  year={2020},
  doi={10.1002/chem.201903525},
  title={The structural basis for processing of unnatural base pairs by DNA polymerases},
  number={16},
  volume={26},
  issn={0947-6539},
  journal={Chemistry - A European Journal},
  pages={3446--3463},
  author={Marx, Andreas and Betz, Karin}
}
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