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Wellenleitermikroskopie : eine neue Methode zur Kraftmessung in biologischen Systemen

Wellenleitermikroskopie : eine neue Methode zur Kraftmessung in biologischen Systemen


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GIEBEL, Karl-Friedrich, 2003. Wellenleitermikroskopie : eine neue Methode zur Kraftmessung in biologischen Systemen [Dissertation]. Konstanz: University of Konstanz

@phdthesis{Giebel2003Welle-4700, title={Wellenleitermikroskopie : eine neue Methode zur Kraftmessung in biologischen Systemen}, year={2003}, author={Giebel, Karl-Friedrich}, address={Konstanz}, school={Universität Konstanz} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/4700"> <dc:contributor>Giebel, Karl-Friedrich</dc:contributor> <dcterms:issued>2003</dcterms:issued> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Giebel, Karl-Friedrich</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T14:49:42Z</dc:date> <dcterms:alternative>Waveguide Microscopy - Forces in Biological Systems</dcterms:alternative> <dc:rights>terms-of-use</dc:rights> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/4700/1/DissertationKOPS.pdf"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T14:49:42Z</dcterms:available> <dc:language>deu</dc:language> <dcterms:abstract xml:lang="eng">In this thesis a new microscopy method is introduced to investigate biological forces, the Waveguide Microscopy (WLM). This optical technique allows to measure forces occuring on substrates in a sub-nanonewton range with microscopic lateral resolution. The core of the method are thin planar waveguides, which are elastically deformable and which are used as substrate. If a local force is applied on such a waveguide, its thickness and therefore its optical resonance frequency changes. By measuring the shift of the optical resonances the local thickness can be obtained; and with the knowledge of the elastic properties of the waveguide the normal force exerted on the substrate can be calculated. In the first part of this work three different optical setups and the waveguide substrates are characterised regarding sensitivity, lateral resolution and mechanical stability. It turns out that the preparation of the planar waveguides is the crucial point of the method. Waveguides comprised of a soft rubber film between two metal layers on a substrate, which are also long-time stable against culture media and biological preparation can be obtained. This offers the possibility of in situ measurements on biological cells. The force sensitivity of the waveguides is better than 20 nanonewton, and the lateral resolution smaller than 3 micron. In the second part of this thesis biological experiments with spores of funghi and adhering cells are presented. Both classes of experiments show not only the applicability of the method to the corresponding force range, but are also relevant in the particular fields of research. In the case of the spores of funghi Colletotrichum graminicola a correlation between the osmotic potential and the capability of leaf penetration could be demonstrated. Besides the influence of different mutants and transformants has been investigated. In particular time-resolved measurement show the WLM as an attractive technique to measure biological forces.</dcterms:abstract> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/4700/1/DissertationKOPS.pdf"/> <dc:format>application/pdf</dc:format> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/41"/> <dcterms:rights rdf:resource="https://kops.uni-konstanz.de/page/termsofuse"/> <dcterms:title>Wellenleitermikroskopie : eine neue Methode zur Kraftmessung in biologischen Systemen</dcterms:title> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/4700"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/41"/> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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