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PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner

PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner

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VEITH, Sebastian, Andrea SCHINK, Marina ENGBRECHT, Matthias MACK, Lisa RANK, Pascal ROSSATTI, Mariam HAKOBYAN, Denise GOLY, Tanja HEFELE, Marco FRENSCH, Arthur FISCHBACH, Alexander BÜRKLE, Aswin MANGERICH, 2019. PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner. In: Scientific Reports. 9(1), 10075. eISSN 2045-2322. Available under: doi: 10.1038/s41598-019-46358-7

@article{Veith2019-12PARP1-46374, title={PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner}, year={2019}, doi={10.1038/s41598-019-46358-7}, number={1}, volume={9}, journal={Scientific Reports}, author={Veith, Sebastian and Schink, Andrea and Engbrecht, Marina and Mack, Matthias and Rank, Lisa and Rossatti, Pascal and Hakobyan, Mariam and Goly, Denise and Hefele, Tanja and Frensch, Marco and Fischbach, Arthur and Bürkle, Alexander and Mangerich, Aswin}, note={Article Number: 10075} }

Hefele, Tanja PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner Fischbach, Arthur Hefele, Tanja Engbrecht, Marina Hakobyan, Mariam Mangerich, Aswin Bürkle, Alexander Goly, Denise Rossatti, Pascal The prime function of nucleoli is ribogenesis, however, several other, non-canonical functions have recently been identified, including a role in genotoxic stress response. Upon DNA damage, numerous proteins shuttle dynamically between the nucleolus and the nucleoplasm, yet the underlying molecular mechanisms are incompletely understood. Here, we demonstrate that PARP1 and PARylation contribute to genotoxic stress-induced nucleolar-nucleoplasmic shuttling of key genome maintenance factors in HeLa cells. Our work revealed that the RECQ helicase, WRN, translocates from nucleoli to the nucleoplasm upon treatment with the oxidizing agent H<sub>2</sub>O<sub>2</sub>, the alkylating agent 2-chloroethyl ethyl sulfide (CEES), and the topoisomerase inhibitor camptothecin (CPT). We show that after treatment with H<sub>2</sub>O<sub>2</sub> and CEES, but not CPT, WRN translocation was dependent on PARP1 protein, yet independent of its enzymatic activity. In contrast, nucleolar-nucleoplasmic translocation of the base excision repair protein, XRCC1, was dependent on both PARP1 protein and its enzymatic activity. Furthermore, gossypol, which inhibits PARP1 activity by disruption of PARP1-protein interactions, abolishes nucleolar-nucleoplasmic shuttling of WRN, XRCC1 and PARP1, indicating the involvement of further upstream factors. In conclusion, this study highlights a prominent role of PARP1 in the DNA damage-induced nucleolar-nucleoplasmic shuttling of genome maintenance factors in HeLa cells in a toxicant and protein-specific manner. Rank, Lisa 2019-07-15T14:38:03Z Veith, Sebastian Hakobyan, Mariam Rossatti, Pascal eng Veith, Sebastian 2019-07-15T14:38:03Z Goly, Denise Engbrecht, Marina Schink, Andrea Frensch, Marco Frensch, Marco Mack, Matthias Rank, Lisa Fischbach, Arthur Mangerich, Aswin terms-of-use 2019-12 Mack, Matthias Schink, Andrea Bürkle, Alexander

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