Synthesis of Erythropoietins Site‐Specifically Conjugated with Complex‐Type N‐Glycans
| Type of Publication: | Journal article |
| Publication status: | Published |
| Author: | Streichert, Katharina; Seitz, Carina; Hoffmann, Eugenia; Boos, Irene; Jelkmann, Wolfgang; Brunner, Thomas; Unverzagt, Carlo; Rubini, Marina |
| Year of publication: | 2019 |
| Published in: | ChemBioChem ; 2019. - ISSN 1439-4227. - eISSN 1439-7633 |
| Pubmed ID: | 30973186 |
| DOI (citable link): | https://dx.doi.org/10.1002/cbic.201900023 |
| Summary: |
The biological activity of glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans. We aimed at readily attaching N-glycans to EPO via defined alkyne groups. EPO variants with an alkyne bearing amino acid (Plk) at the N-glycosylation sites 24, 38 and 83 were obtained by amber suppression followed by refolding. Click-conjugation of the alkinyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.
|
| Subject (DDC): | 540 Chemistry |
| Keywords: | Click chemistry, Glycoproteins, Protein Engineering, Protein modifications, synthetic glycans |
| Bibliography of Konstanz: | Yes |
| Refereed: | Yes |
| Online First: Journal articles that are published online before they appear as an actual part of a journal issue. Online first articles are published on the journal's website in the publisher's version. | |
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STREICHERT, Katharina, Carina SEITZ, Eugenia HOFFMANN, Irene BOOS, Wolfgang JELKMANN, Thomas BRUNNER, Carlo UNVERZAGT, Marina RUBINI, 2019. Synthesis of Erythropoietins Site‐Specifically Conjugated with Complex‐Type N‐Glycans. In: ChemBioChem. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201900023
@article{Streichert2019-04-11Synth-46286, title={Synthesis of Erythropoietins Site‐Specifically Conjugated with Complex‐Type N‐Glycans}, year={2019}, doi={10.1002/cbic.201900023}, issn={1439-4227}, journal={ChemBioChem}, author={Streichert, Katharina and Seitz, Carina and Hoffmann, Eugenia and Boos, Irene and Jelkmann, Wolfgang and Brunner, Thomas and Unverzagt, Carlo and Rubini, Marina} }
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