Synthesis of Erythropoietins Site‐Specifically Conjugated with Complex‐Type N‐Glycans
| Type of Publication: | Journal article |
| Publication status: | Published |
| URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-2-1k6u2xu7jov4m7 |
| Author: | Streichert, Katharina; Seitz, Carina; Hoffmann, Eugenia; Boos, Irene; Jelkmann, Wolfgang; Brunner, Thomas; Unverzagt, Carlo; Rubini, Marina |
| Year of publication: | 2019 |
| Published in: | ChemBioChem ; 20 (2019), 15. - Wiley. - ISSN 1439-4227. - eISSN 1439-7633 |
| Pubmed ID: | 30973186 |
| DOI (citable link): | https://dx.doi.org/10.1002/cbic.201900023 |
| Summary: |
The biological activity of glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans. We aimed at readily attaching N-glycans to EPO via defined alkyne groups. EPO variants with an alkyne bearing amino acid (Plk) at the N-glycosylation sites 24, 38 and 83 were obtained by amber suppression followed by refolding. Click-conjugation of the alkinyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.
|
| Subject (DDC): | 540 Chemistry |
| Keywords: | Click chemistry, Glycoproteins, Protein Engineering, Protein modifications, synthetic glycans |
| Link to License: | In Copyright |
| Bibliography of Konstanz: | Yes |
| Refereed: | Yes |
Cite This
Files in this item
![[PDF]](/themes/Kops/images/mimes/pdf.png "PDF file"){kind=small}
STREICHERT, Katharina, Carina SEITZ, Eugenia HOFFMANN, Irene BOOS, Wolfgang JELKMANN, Thomas BRUNNER, Carlo UNVERZAGT, Marina RUBINI, 2019. Synthesis of Erythropoietins Site‐Specifically Conjugated with Complex‐Type N‐Glycans. In: ChemBioChem. Wiley. 20(15). ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201900023
@article{Streichert2019-08-01Synth-46286, title={Synthesis of Erythropoietins Site‐Specifically Conjugated with Complex‐Type N‐Glycans}, year={2019}, doi={10.1002/cbic.201900023}, number={15}, volume={20}, issn={1439-4227}, journal={ChemBioChem}, author={Streichert, Katharina and Seitz, Carina and Hoffmann, Eugenia and Boos, Irene and Jelkmann, Wolfgang and Brunner, Thomas and Unverzagt, Carlo and Rubini, Marina} }
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/46286"> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:abstract xml:lang="eng">The biological activity of glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans. We aimed at readily attaching N-glycans to EPO via defined alkyne groups. EPO variants with an alkyne bearing amino acid (Plk) at the N-glycosylation sites 24, 38 and 83 were obtained by amber suppression followed by refolding. Click-conjugation of the alkinyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.</dcterms:abstract> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Brunner, Thomas</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-07-10T09:34:49Z</dcterms:available> <dc:contributor>Hoffmann, Eugenia</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Jelkmann, Wolfgang</dc:contributor> <dc:contributor>Boos, Irene</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Seitz, Carina</dc:contributor> <dc:creator>Rubini, Marina</dc:creator> <dc:contributor>Unverzagt, Carlo</dc:contributor> <dc:contributor>Streichert, Katharina</dc:contributor> <dc:contributor>Rubini, Marina</dc:contributor> <dcterms:issued>2019-08-01</dcterms:issued> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/46286"/> <dc:creator>Jelkmann, Wolfgang</dc:creator> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/46286/1/Streichert_2-1k6u2xu7jov4m7.pdf"/> <dcterms:title>Synthesis of Erythropoietins Site‐Specifically Conjugated with Complex‐Type N‐Glycans</dcterms:title> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-07-10T09:34:49Z</dc:date> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/46286/1/Streichert_2-1k6u2xu7jov4m7.pdf"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:creator>Seitz, Carina</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Hoffmann, Eugenia</dc:creator> <dc:contributor>Brunner, Thomas</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:creator>Unverzagt, Carlo</dc:creator> <dc:creator>Streichert, Katharina</dc:creator> <dc:creator>Boos, Irene</dc:creator> <dc:rights>terms-of-use</dc:rights> </rdf:Description> </rdf:RDF>
Downloads since Jul 10, 2019 (Information about access statistics)
| Streichert_2-1k6u2xu7jov4m7.pdf | 227 |
This item appears in the following Collection(s)
Search KOPS
Browse
My Account
