Functionalized DNA Hydrogels Produced by Polymerase-Catalyzed Incorporation of Non-Natural Nucleotides as a Surface Coating for Cell Culture Applications

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2019
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Schneider, Ann-Kathrin
Niemeyer, Christof M.
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Advanced Healthcare Materials. 2019, 8(9), 1900080. ISSN 2192-2640. eISSN 2192-2659. Available under: doi: 10.1002/adhm.201900080
Zusammenfassung

Cells from most mammalian tissues require an extracellular matrix (ECM) for attachment and proper functioning. In vitro cell cultures therefore must be supplied with an ECM that satisfies both the biological needs of cells used and the technical demands of the experimental setup. The latter include matrix functionalization for cell attachment, favorable microscopic properties, and affordable production costs. Here, modified DNA materials are therefore developed as an ECM mimic. The material is prepared by chemical cross-linking of commonly available salmon sperm DNA. To render the material cell-compatible, it is enzymatically modified by DNA polymerase I to provide versatile attachment points for peptides, proteins, or antibodies via a modular strategy. Different cells specifically attach to the material, even from mixed populations. They can be mildly released for further cell studies by DNase I-mediated digestion of the DNA material. Additionally, neural stem cells not only attach and survive on the material but also differentiate to a neural lineage when prompted. Furthermore, the DNA material can be employed to capture and retain cells under flow conditions. The simple preparation of the DNA material and its wide scope of applications open new perspectives for various cell study challenges and medical applications.

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ISO 690FINKE, Alexander, Ann-Kathrin SCHNEIDER, Anna-Sophie SPRENG, Marcel LEIST, Christof M. NIEMEYER, Andreas MARX, 2019. Functionalized DNA Hydrogels Produced by Polymerase-Catalyzed Incorporation of Non-Natural Nucleotides as a Surface Coating for Cell Culture Applications. In: Advanced Healthcare Materials. 2019, 8(9), 1900080. ISSN 2192-2640. eISSN 2192-2659. Available under: doi: 10.1002/adhm.201900080
BibTex
@article{Finke2019-05Funct-45752,
  year={2019},
  doi={10.1002/adhm.201900080},
  title={Functionalized DNA Hydrogels Produced by Polymerase-Catalyzed Incorporation of Non-Natural Nucleotides as a Surface Coating for Cell Culture Applications},
  number={9},
  volume={8},
  issn={2192-2640},
  journal={Advanced Healthcare Materials},
  author={Finke, Alexander and Schneider, Ann-Kathrin and Spreng, Anna-Sophie and Leist, Marcel and Niemeyer, Christof M. and Marx, Andreas},
  note={Article Number: 1900080}
}
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