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Kinky DNA in solution : Small-angle-scattering study of a nucleosome positioning sequence

Kinky DNA in solution : Small-angle-scattering study of a nucleosome positioning sequence

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SCHINDLER, Torben, Adrián GONZÁLEZ, Ramachandran BOOPATHI, Marta Marty RODA, Lorena ROMERO-SANTACREU, Andrew WILDES, Lionel PORCAR, Anne MARTEL, Nikos THEODORAKOPOULOS, Michel PEYRARD, 2018. Kinky DNA in solution : Small-angle-scattering study of a nucleosome positioning sequence. In: Physical Review E. 98(4), 042417. ISSN 2470-0045. eISSN 2470-0053. Available under: doi: 10.1103/PhysRevE.98.042417

@article{Schindler2018Kinky-45161, title={Kinky DNA in solution : Small-angle-scattering study of a nucleosome positioning sequence}, year={2018}, doi={10.1103/PhysRevE.98.042417}, number={4}, volume={98}, issn={2470-0045}, journal={Physical Review E}, author={Schindler, Torben and González, Adrián and Boopathi, Ramachandran and Roda, Marta Marty and Romero-Santacreu, Lorena and Wildes, Andrew and Porcar, Lionel and Martel, Anne and Theodorakopoulos, Nikos and Peyrard, Michel}, note={Article Number: 042417} }

González, Adrián Schindler, Torben Boopathi, Ramachandran Kinky DNA in solution : Small-angle-scattering study of a nucleosome positioning sequence Theodorakopoulos, Nikos Wildes, Andrew Boopathi, Ramachandran Roda, Marta Marty 2019-02-22T13:16:53Z DNA is a flexible molecule, but the degree of its flexibility is subject to debate. The commonly-accepted persistence length of l<sub>p</sub>≈500Å is inconsistent with recent studies on short-chain DNA that show much greater flexibility but do not probe its origin. We have performed x-ray and neutron small-angle scattering on a short DNA sequence containing a strong nucleosome positioning element and analyzed the results using a modified Kratky-Porod model to determine possible conformations. Our results support a hypothesis from Crick and Klug in 1975 that some DNA sequences in solution can have sharp kinks, potentially resolving the discrepancy. Our conclusions are supported by measurements on a radiation-damaged sample, where single-strand breaks lead to increased flexibility and by an analysis of data from another sequence, which does not have kinks, but where our method can detect a locally enhanced flexibility due to an AT domain. eng 2018 Romero-Santacreu, Lorena Roda, Marta Marty Schindler, Torben González, Adrián Romero-Santacreu, Lorena Porcar, Lionel Theodorakopoulos, Nikos Wildes, Andrew Peyrard, Michel Martel, Anne 2019-02-22T13:16:53Z Porcar, Lionel Martel, Anne Peyrard, Michel

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