Direct Imaging of Protein‐Specific Methylation in Mammalian Cells

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DOLL, Franziska, Raphael STEIMBACH, Andreas ZUMBUSCH, 2019. Direct Imaging of Protein‐Specific Methylation in Mammalian Cells. In: Chembiochem. 20(10), pp. 1315-1325. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201800787

@article{Doll2019-05-15Direc-44865, title={Direct Imaging of Protein‐Specific Methylation in Mammalian Cells}, year={2019}, doi={10.1002/cbic.201800787}, number={10}, volume={20}, issn={1439-4227}, journal={Chembiochem}, pages={1315--1325}, author={Doll, Franziska and Steimbach, Raphael and Zumbusch, Andreas} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dcterms:issued>2019-05-15</dcterms:issued> <dc:creator>Zumbusch, Andreas</dc:creator> <dcterms:available rdf:datatype="">2019-02-05T14:58:14Z</dcterms:available> <dcterms:abstract xml:lang="eng">The abundant post-translational modification methylation alters a protein's function, stability, and/or localization. Its malfunctions are associated with severe diseases. To unravel protein methylation sites and their biological functions, chemical methylation reporters have been developed. However, until now their usage was limited to cell lysates. Here, we present the first generally applicable approach for imaging methylation of individual proteins in human cells, which is based on a combination of chemical reporter strategies, bioorthogonal ligation reactions, and Förster resonance energy transfer (FRET) detected by fluorescence lifetime imaging (FLIM) microscopy. Using this approach, we succeeded in imaging methylation of histone 4 and the non-histone proteins tumor suppressor p53, kinase Akt1, and transcription factor Foxo1 in two human cell lines. To further demonstrate its potential we visualized the localization-dependent methylation state of Foxo1 in the cellular context.</dcterms:abstract> <dc:contributor>Zumbusch, Andreas</dc:contributor> <dcterms:title>Direct Imaging of Protein‐Specific Methylation in Mammalian Cells</dcterms:title> <dc:language>eng</dc:language> <dspace:isPartOfCollection rdf:resource=""/> <bibo:uri rdf:resource=""/> <dc:creator>Steimbach, Raphael</dc:creator> <dc:date rdf:datatype="">2019-02-05T14:58:14Z</dc:date> <dc:contributor>Doll, Franziska</dc:contributor> <dc:creator>Doll, Franziska</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:isPartOf rdf:resource=""/> <dc:contributor>Steimbach, Raphael</dc:contributor> </rdf:Description> </rdf:RDF>

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