Preparation and Application of Enzyme-Nucleotide Conjugates

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WELTER, Moritz, Andreas MARX, 2018. Preparation and Application of Enzyme-Nucleotide Conjugates. In: Current protocols in chemical biology. 10(1), pp. 49-71. eISSN 2160-4762. Available under: doi: 10.1002/cpch.36

@article{Welter2018Prepa-44590, title={Preparation and Application of Enzyme-Nucleotide Conjugates}, year={2018}, doi={10.1002/cpch.36}, number={1}, volume={10}, journal={Current protocols in chemical biology}, pages={49--71}, author={Welter, Moritz and Marx, Andreas} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/44590"> <dcterms:issued>2018</dcterms:issued> <dc:contributor>Welter, Moritz</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:language>eng</dc:language> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:creator>Welter, Moritz</dc:creator> <dcterms:title>Preparation and Application of Enzyme-Nucleotide Conjugates</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-01-16T14:02:33Z</dcterms:available> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/44590"/> <dc:contributor>Marx, Andreas</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Marx, Andreas</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-01-16T14:02:33Z</dc:date> <dcterms:abstract xml:lang="eng">In this unit the preparation and application of enzyme-nucleotide conjugates is depicted. First, a modified nucleoside triphosphate is synthesized bearing a long and flexible linker equipped with a thiol group. The nucleotide is then reacted with maleimide-activated horseradish peroxidase to yield an enzyme-nucleotide conjugate, which due to the long linker, can be used as a substrate by DNA polymerases in primer extension reactions. Finally, an assay based on these findings is described that provides a fast and easy nucleic acid detection and genotyping platform.</dcterms:abstract> </rdf:Description> </rdf:RDF>

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