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Initiation of RNA synthesis by the hepatitis C virus RNA-dependent RNA polymerase is affected by the structure of the RNA template

Initiation of RNA synthesis by the hepatitis C virus RNA-dependent RNA polymerase is affected by the structure of the RNA template

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REICH, Stefan, Michael KOVERMANN, Hauke LILIE, Paul KNICK, René GEISSLER, Ralph Peter GOLBIK, Jochen BALBACH, Sven-Erik BEHRENS, 2014. Initiation of RNA synthesis by the hepatitis C virus RNA-dependent RNA polymerase is affected by the structure of the RNA template. In: Biochemistry. 53(44), pp. 7002-7012. ISSN 0006-2960. eISSN 1520-4995. Available under: doi: 10.1021/bi5006656

@article{Reich2014-11-11Initi-44422, title={Initiation of RNA synthesis by the hepatitis C virus RNA-dependent RNA polymerase is affected by the structure of the RNA template}, year={2014}, doi={10.1021/bi5006656}, number={44}, volume={53}, issn={0006-2960}, journal={Biochemistry}, pages={7002--7012}, author={Reich, Stefan and Kovermann, Michael and Lilie, Hauke and Knick, Paul and Geissler, René and Golbik, Ralph Peter and Balbach, Jochen and Behrens, Sven-Erik} }

Golbik, Ralph Peter Lilie, Hauke Kovermann, Michael Behrens, Sven-Erik 2014-11-11 2019-01-07T12:01:49Z Lilie, Hauke 2019-01-07T12:01:49Z Balbach, Jochen Knick, Paul The hepatitis C virus (HCV) RNA-dependent RNA polymerase NS5B is a central enzyme of the intracellular replication of the viral (+)RNA genome. Here, we studied the individual steps of NS5B-catalyzed RNA synthesis by a combination of biophysical methods, including real-time 1D <sup>1</sup>H NMR spectroscopy. NS5B was found to bind to a nonstructured and a structured RNA template in different modes. Following NTP binding and conversion to the catalysis-competent ternary complex, the polymerase revealed an improved affinity for the template. By monitoring the folding/unfolding of 3'(-)SL by <sup>1</sup>H NMR, the base pair at the stem's edge was identified as the most stable component of the structure. <sup>1</sup>H NMR real-time analysis of NS5B-catalyzed RNA synthesis on 3'(-)SL showed that a pronounced lag phase preceded the processive polymerization reaction. The presence of the double-stranded stem with the edge base pair acting as the main energy barrier impaired RNA synthesis catalyzed by NS5B. Our observations suggest a crucial role of RNA-modulating factors in the HCV replication process. Reich, Stefan Knick, Paul Behrens, Sven-Erik Geissler, René Geissler, René Balbach, Jochen Initiation of RNA synthesis by the hepatitis C virus RNA-dependent RNA polymerase is affected by the structure of the RNA template Kovermann, Michael Golbik, Ralph Peter eng Reich, Stefan

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