KOPS - The Institutional Repository of the University of Konstanz

Widespread bacterial lysine degradation proceeding via glutarate and L-2-hydroxyglutarate

Widespread bacterial lysine degradation proceeding via glutarate and L-2-hydroxyglutarate

Cite This

Files in this item

Checksum: MD5:c3b9272c386d406152bf7b8f1bec3f59

KNORR, Sebastian, Malte SINN, Dmitry GALETSKIY, Rhys M. WILLIAMS, Changhao WANG, Nicolai MÜLLER, Olga MAYANS, David SCHLEHECK, Jörg S. HARTIG, 2018. Widespread bacterial lysine degradation proceeding via glutarate and L-2-hydroxyglutarate. In: Nature Communications. 9(1), 5071. eISSN 2041-1723. Available under: doi: 10.1038/s41467-018-07563-6

@article{Knorr2018Wides-44395, title={Widespread bacterial lysine degradation proceeding via glutarate and L-2-hydroxyglutarate}, year={2018}, doi={10.1038/s41467-018-07563-6}, number={1}, volume={9}, journal={Nature Communications}, author={Knorr, Sebastian and Sinn, Malte and Galetskiy, Dmitry and Williams, Rhys M. and Wang, Changhao and Müller, Nicolai and Mayans, Olga and Schleheck, David and Hartig, Jörg S.}, note={Article Number: 5071} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/44395"> <dcterms:rights rdf:resource="https://kops.uni-konstanz.de/page/termsofuse"/> <dc:contributor>Wang, Changhao</dc:contributor> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/44395/3/Knorr_2-1hxbevif2xpaf4.pdf"/> <dc:creator>Sinn, Malte</dc:creator> <dc:contributor>Galetskiy, Dmitry</dc:contributor> <dc:creator>Mayans, Olga</dc:creator> <dc:language>eng</dc:language> <dc:creator>Hartig, Jörg S.</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-12-19T15:12:56Z</dc:date> <dc:creator>Schleheck, David</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-12-19T15:12:56Z</dcterms:available> <dc:rights>terms-of-use</dc:rights> <dc:creator>Williams, Rhys M.</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Knorr, Sebastian</dc:creator> <dc:creator>Galetskiy, Dmitry</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/44395"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:contributor>Williams, Rhys M.</dc:contributor> <dc:creator>Wang, Changhao</dc:creator> <dc:contributor>Hartig, Jörg S.</dc:contributor> <dc:contributor>Knorr, Sebastian</dc:contributor> <dc:contributor>Schleheck, David</dc:contributor> <dc:creator>Müller, Nicolai</dc:creator> <dc:contributor>Mayans, Olga</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/44395/3/Knorr_2-1hxbevif2xpaf4.pdf"/> <dc:contributor>Sinn, Malte</dc:contributor> <dcterms:issued>2018</dcterms:issued> <dcterms:title>Widespread bacterial lysine degradation proceeding via glutarate and L-2-hydroxyglutarate</dcterms:title> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Müller, Nicolai</dc:contributor> <dcterms:abstract xml:lang="eng">Lysine degradation has remained elusive in many organisms including Escherichia coli. Here we report catabolism of lysine to succinate in E. coli involving glutarate and L-2-hydroxyglutarate as intermediates. We show that CsiD acts as an α-ketoglutarate-dependent dioxygenase catalysing hydroxylation of glutarate to L-2-hydroxyglutarate. CsiD is found widespread in bacteria. We present crystal structures of CsiD in complex with glutarate, succinate, and the inhibitor N-oxalyl-glycine, demonstrating strong discrimination between the structurally related ligands. We show that L-2-hydroxyglutarate is converted to α-ketoglutarate by LhgO acting as a membrane-bound, ubiquinone-linked dehydrogenase. Lysine enters the pathway via 5-aminovalerate by the promiscuous enzymes GabT and GabD. We demonstrate that repression of the pathway by CsiR is relieved upon glutarate binding. In conclusion, lysine degradation provides an important link in central metabolism. Our results imply the gut microbiome as a potential source of glutarate and L-2-hydroxyglutarate associated with human diseases such as cancer and organic acidurias.</dcterms:abstract> </rdf:Description> </rdf:RDF>

Downloads since Dec 19, 2018 (Information about access statistics)

Knorr_2-1hxbevif2xpaf4.pdf 194

This item appears in the following Collection(s)

Search KOPS


Browse

My Account