Fast and accurate data collection for macromolecular crystallography using the JUNGFRAU detector

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2018
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Leonarski, Filip
Redford, Sophie
Mozzanica, Aldo
Lopez-Cuenca, Carlos
Panepucci, Ezequiel
Nass, Karol
Schmitt, Bernd
Wang, Meitian
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Nature methods. 2018, 15(10), pp. 799-804. ISSN 1548-7091. eISSN 1548-7105. Available under: doi: 10.1038/s41592-018-0143-7
Zusammenfassung

The accuracy of X-ray diffraction data is directly related to how the X-ray detector records photons. Here we describe the application of a direct-detection charge-integrating pixel-array detector (JUNGFRAU) in macromolecular crystallography (MX). JUNGFRAU features a uniform response on the subpixel level, linear behavior toward high photon rates, and low-noise performance across the whole dynamic range. We demonstrate that these features allow accurate MX data to be recorded at unprecedented speed. We also demonstrate improvements over previous-generation detectors in terms of data quality, using native single-wavelength anomalous diffraction (SAD) phasing, for thaumatin, lysozyme, and aminopeptidase N. Our results suggest that the JUNGFRAU detector will substantially improve the performance of synchrotron MX beamlines and equip them for future synchrotron light sources.

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ISO 690LEONARSKI, Filip, Sophie REDFORD, Aldo MOZZANICA, Carlos LOPEZ-CUENCA, Ezequiel PANEPUCCI, Karol NASS, Roman SCHNEIDER, Kay DIEDERICHS, Bernd SCHMITT, Meitian WANG, 2018. Fast and accurate data collection for macromolecular crystallography using the JUNGFRAU detector. In: Nature methods. 2018, 15(10), pp. 799-804. ISSN 1548-7091. eISSN 1548-7105. Available under: doi: 10.1038/s41592-018-0143-7
BibTex
@article{Leonarski2018-10accur-43974,
  year={2018},
  doi={10.1038/s41592-018-0143-7},
  title={Fast and accurate data collection for macromolecular crystallography using the JUNGFRAU detector},
  number={10},
  volume={15},
  issn={1548-7091},
  journal={Nature methods},
  pages={799--804},
  author={Leonarski, Filip and Redford, Sophie and Mozzanica, Aldo and Lopez-Cuenca, Carlos and Panepucci, Ezequiel and Nass, Karol and Schneider, Roman and Diederichs, Kay and Schmitt, Bernd and Wang, Meitian}
}
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