Quantitative analysis of three-dimensional fluorescence localization microscopy data
| Type of Publication: | Journal article |
| Publication status: | Published |
| Author: | Owen, Dylan M.; Williamson, David J.; Boelen, Lies; Magenau, Astrid; Rossy, Jérémie; Gaus, Katharina |
| Year of publication: | 2013 |
| Published in: | Biophysical journal ; 105 (2013), 2. - pp. L05-L07. - ISSN 0006-3495. - eISSN 1542-0086 |
| Pubmed ID: | 23870275 |
| DOI (citable link): | https://dx.doi.org/10.1016/j.bpj.2013.05.063 |
| Summary: |
Identifying the three-dimensional molecular organization of subcellular organelles in intact cells has been challenging to date. Here we present an analysis approach for three-dimensional localization microscopy that can not only identify subcellular objects below the diffraction limit but also quantify their shape and volume. This approach is particularly useful to map the topography of the plasma membrane and measure protein distribution within an undulating membrane.
|
| Subject (DDC): | 570 Biosciences, Biology |
| Refereed: | Yes |
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OWEN, Dylan M., David J. WILLIAMSON, Lies BOELEN, Astrid MAGENAU, Jérémie ROSSY, Katharina GAUS, 2013. Quantitative analysis of three-dimensional fluorescence localization microscopy data. In: Biophysical journal. 105(2), pp. L05-L07. ISSN 0006-3495. eISSN 1542-0086. Available under: doi: 10.1016/j.bpj.2013.05.063
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