A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation

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2018
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Compeer, Ewoud B.
Kraus, Felix
Ecker, Manuela
Redpath, Gregory
Amiezer, Mayan
Rother, Nils
Nicovich, Philip R.
Kapoor-Kaushik, Natasha
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Nature Communications ; 9 (2018). - 1597. - eISSN 2041-1723
Abstract
Endocytosis of surface receptors and their polarized recycling back to the plasma membrane are central to many cellular processes, such as cell migration, cytokinesis, basolateral polarity of epithelial cells and T cell activation. Little is known about the mechanisms that control the organization of recycling endosomes and how they connect to receptor endocytosis. Here, we follow the endocytic journey of the T cell receptor (TCR), from internalization at the plasma membrane to recycling back to the immunological synapse. We show that TCR triggering leads to its rapid uptake through a clathrin-independent pathway. Immediately after internalization, TCR is incorporated into a mobile and long-lived endocytic network demarked by the membrane-organizing proteins flotillins. Although flotillins are not required for TCR internalization, they are necessary for its recycling to the immunological synapse. We further show that flotillins are essential for T cell activation, supporting TCR nanoscale organization and signaling.
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570 Biosciences, Biology
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Cite This
ISO 690COMPEER, Ewoud B., Felix KRAUS, Manuela ECKER, Gregory REDPATH, Mayan AMIEZER, Nils ROTHER, Philip R. NICOVICH, Natasha KAPOOR-KAUSHIK, Guerric P. B. SAMSON, Jérémie ROSSY, 2018. A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation. In: Nature Communications. 9, 1597. eISSN 2041-1723. Available under: doi: 10.1038/s41467-018-04088-w
BibTex
@article{Compeer2018-04-23mobil-42957,
  year={2018},
  doi={10.1038/s41467-018-04088-w},
  title={A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation},
  volume={9},
  journal={Nature Communications},
  author={Compeer, Ewoud B. and Kraus, Felix and Ecker, Manuela and Redpath, Gregory and Amiezer, Mayan and Rother, Nils and Nicovich, Philip R. and Kapoor-Kaushik, Natasha and Samson, Guerric P. B. and Rossy, Jérémie},
  note={Article Number: 1597}
}
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