KOPS - The Institutional Repository of the University of Konstanz

A combined approach of surface passivation and specific immobilization to study biomolecules by ATR-FTIR spectroscopy

Aufgrund von Vorbereitungen auf eine neue Version von KOPS, können kommenden Montag und Dienstag keine Publikationen eingereicht werden. (Due to preparations for a new version of KOPS, no publications can be submitted next Monday and Tuesday.)

A combined approach of surface passivation and specific immobilization to study biomolecules by ATR-FTIR spectroscopy

Cite This

Files in this item

Checksum: MD5:10a36efd3bbdd699da5940f76218ccaa

KRÜGER, Annika, Alexander BÜRKLE, Aswin MANGERICH, Karin HAUSER, 2018. A combined approach of surface passivation and specific immobilization to study biomolecules by ATR-FTIR spectroscopy. In: Biomedical Spectroscopy and Imaging. 7(1-2), pp. 25-33. ISSN 2212-8794. eISSN 2212-8808. Available under: doi: 10.3233/BSI-180174

@article{Kruger2018Acomb-42893, title={A combined approach of surface passivation and specific immobilization to study biomolecules by ATR-FTIR spectroscopy}, year={2018}, doi={10.3233/BSI-180174}, number={1-2}, volume={7}, issn={2212-8794}, journal={Biomedical Spectroscopy and Imaging}, pages={25--33}, author={Krüger, Annika and Bürkle, Alexander and Mangerich, Aswin and Hauser, Karin} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/42893"> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/52"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Hauser, Karin</dc:contributor> <dc:contributor>Bürkle, Alexander</dc:contributor> <dc:creator>Mangerich, Aswin</dc:creator> <dc:contributor>Krüger, Annika</dc:contributor> <dc:creator>Bürkle, Alexander</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/42893/3/Krueger_2-dkp7rgpmx9h07.pdf"/> <dcterms:abstract xml:lang="eng">Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy is a surface-sensitive and label-free technique, which is applied to obtain dynamic structural information of biomolecules. The study of proteins by ATR-FTIR spectroscopy can be impeded by their tendency to adsorb to solid surfaces. Furthermore, the adsorption process of proteins is often accompanied with conformational changes, which can interfere with the intended structural analysis. We efficiently modified a silicon ATR crystal surface with polyethylene glycol and thereby create a protein-repellent surface. To achieve a high sensitivity, which enables the study of small conformational changes of biomolecules, we combine surface passivation with specific immobilization. This is accomplished via the biotin-streptavidin interaction, which is one of the strongest known non-covalent protein-ligand interactions. As a proof of concept we present the specific immobilization of DNA. The modified surface is stable against elevated temperatures and 8 M urea and can therefore be used to study a wide range of biochemical systems and reactions. The surface chemistry is simple and performed under mild conditions, which leads to a high applicability of the presented approach.</dcterms:abstract> <dc:language>eng</dc:language> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:creator>Krüger, Annika</dc:creator> <dcterms:title>A combined approach of surface passivation and specific immobilization to study biomolecules by ATR-FTIR spectroscopy</dcterms:title> <dcterms:issued>2018</dcterms:issued> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-07-20T08:28:46Z</dc:date> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/52"/> <dc:contributor>Mangerich, Aswin</dc:contributor> <dc:creator>Hauser, Karin</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/42893"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/42893/3/Krueger_2-dkp7rgpmx9h07.pdf"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-07-20T08:28:46Z</dcterms:available> </rdf:Description> </rdf:RDF>

Downloads since Jul 20, 2018 (Information about access statistics)

Krueger_2-dkp7rgpmx9h07.pdf 354

This item appears in the following Collection(s)

Search KOPS


Browse

My Account