Aufgrund von Vorbereitungen auf eine neue Version von KOPS, können am Montag, 6.2. und Dienstag, 7.2. keine Publikationen eingereicht werden. (Due to preparations for a new version of KOPS, no publications can be submitted on Monday, Feb. 6 and Tuesday, Feb. 7.)
Type of Publication: | Journal article |
Publication status: | Published |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-2-15ij7j0tgdvgz6 |
Author: | Schmalz, Michael F.; Wieser, Ines; Schindler, Felix; Czada, Christina; Leitenstorfer, Alfred; Ferrando-May, Elisa |
Year of publication: | 2018 |
Published in: | Optics Letters ; 43 (2018), 12. - pp. 2877-2880. - ISSN 0146-9592. - eISSN 1539-4794 |
DOI (citable link): | https://dx.doi.org/10.1364/OL.43.002877 |
Summary: |
We present a three-color femtosecond Er/Yb:fiber laser enabling highly specific and standardized nonlinear optical manipulation of live cells. The system simultaneously provides bandwidth-limited 80-fs pulses with identical intensity envelope centered at wavelengths of 515, 775, and 1035 nm in the focus of a confocal microscope. We achieve this goal by combining high-order dispersion control via, for example, chirped fiber Bragg gratings with proper bandwidth management in each nonlinear conversion step. Wavelength-selective and noninterfering induction of deoxyribonucleic acid (DNA) photoproducts and DNA strand breaks, as well as fluorescence photoactivation of a photoactivatable green fluorescent protein (PA-GFP)–histone fusion protein, are demonstrated. The capability to introduce different types of DNA lesions and perform photoswitching experiments in a selective manner is essential for quantitative studies on DNA repair and chromatin dynamics.
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Subject (DDC): | 530 Physics |
Link to License: | In Copyright |
Bibliography of Konstanz: | Yes |
Refereed: | Yes |
SCHMALZ, Michael F., Ines WIESER, Felix SCHINDLER, Christina CZADA, Alfred LEITENSTORFER, Elisa FERRANDO-MAY, 2018. Highly standardized multicolor femtosecond fiber system for selective microphotomanipulation of deoxyribonucleic acid and chromatin. In: Optics Letters. 43(12), pp. 2877-2880. ISSN 0146-9592. eISSN 1539-4794. Available under: doi: 10.1364/OL.43.002877
@article{Schmalz2018-06-15Highl-42850, title={Highly standardized multicolor femtosecond fiber system for selective microphotomanipulation of deoxyribonucleic acid and chromatin}, year={2018}, doi={10.1364/OL.43.002877}, number={12}, volume={43}, issn={0146-9592}, journal={Optics Letters}, pages={2877--2880}, author={Schmalz, Michael F. and Wieser, Ines and Schindler, Felix and Czada, Christina and Leitenstorfer, Alfred and Ferrando-May, Elisa} }
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