Type of Publication: | Journal article |
Publication status: | Published |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-2-f8cp9lug6vhd7 |
Author: | Scheffner, Martin; Wessel, Rainer; Stahl, Hans |
Year of publication: | 1989 |
Published in: | Nucleic Acids Research ; 17 (1989), 1. - pp. 93-106. - ISSN 0301-5610. - eISSN 1362-4962 |
DOI (citable link): | https://dx.doi.org/10.1093/nar/17.1.93 |
Summary: |
Simian virus 40 (SV40) large tumor antigen (T antigen) is mainly localized in the nucleus where it exhibits two biochemical properties: DNA binding and helicase activity. Both activities are necessary for viral DNA replication and may also enable T antigen to modulate cellular growth. Here we present biochemical and electron microscopic evidence that the helicase activity can start at internal sites of fully double-stranded DNA molecules not containing the SV40 origin or replication. Using T antigen specific monoclonal antibodies, this unwinding reaction can be biochemically divided in an initiation (duplex opening) and a propagation step. The duplex opening reaction (as well as the propagation step) does not depend on a specific DNA sequence or secondary structure. In addition, we have found that T antigen forms an ATP dependent nucleoprotein complex at double-stranded DNA, which may be an essential step for the sequence independent duplex DNA opening reaction.
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Subject (DDC): | 570 Biosciences, Biology |
Link to License: | In Copyright |
SCHEFFNER, Martin, Rainer WESSEL, Hans STAHL, 1989. Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen. In: Nucleic Acids Research. 17(1), pp. 93-106. ISSN 0301-5610. eISSN 1362-4962. Available under: doi: 10.1093/nar/17.1.93
@article{Scheffner1989Seque-42843, title={Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen}, year={1989}, doi={10.1093/nar/17.1.93}, number={1}, volume={17}, issn={0301-5610}, journal={Nucleic Acids Research}, pages={93--106}, author={Scheffner, Martin and Wessel, Rainer and Stahl, Hans} }
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