A novel structural effector from rust fungi is capable of fibril formation

Kemen, Eric; Kemen, Ariane; Ehlers, Andreas; Voegele, Ralf T.; Mendgen, Kurt

A novel structural effector from rust fungi is capable of fibril formation

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Date

2013

Authors

Kemen, Eric

Kemen, Ariane

Ehlers, Andreas

Voegele, Ralf T.

Mendgen, Kurt

DOI (citable link)

10.1111/tpj.12237

Collections

Biologie

Publication type

Journal article

Publication status

Published

Published in

The Plant Journal ; 75 (2013), 5. - pp. 767-780. - ISSN 0960-7412. - eISSN 1365-313X

Abstract

It has been reported that filament‐forming surface proteins such as hydrophobins are important virulence determinants in fungi and are secreted during pathogenesis. Such proteins have not yet been identified in obligate biotrophic pathogens such as rust fungi. Rust transferred protein 1 (RTP1p), a rust protein that is transferred into the host cytoplasm, accumulates around the haustorial complex. To investigate RTP1p structure and function, we used immunocytological, biochemical and computational approaches. We found that RTP1p accumulates in protuberances of the extra‐haustorial matrix, a compartment that surrounds the haustorium and is separated from the plant cytoplasm by a modified host plasma membrane. Our analyses show that RTP1p is capable of forming filamentous structures in vitro and in vivo. We present evidence that filament formation is due to β–aggregation similar to what has been observed for amyloid‐like proteins. Our findings reveal that RTP1p is a member of a new class of structural effectors. We hypothesize that RTP1p is transferred into the host to stabilize the host cell and protect the haustorium from degradation in later stages of the interaction. Thus, we provide evidence for transfer of an amyloid‐like protein into the host cell, which has potential for the development of new resistance mechanisms against rust fungi.

Subject (DDC)

570 Biosciences, Biology

Cite This

ISO 690

KEMEN, Eric, Ariane KEMEN, Andreas EHLERS, Ralf T. VOEGELE, Kurt MENDGEN, 2013. A novel structural effector from rust fungi is capable of fibril formation. In: The Plant Journal. 75(5), pp. 767-780. ISSN 0960-7412. eISSN 1365-313X. Available under: doi: 10.1111/tpj.12237

BibTex

@article{Kemen2013-09novel-42429,
  year={2013},
  doi={10.1111/tpj.12237},
  title={A novel structural effector from rust fungi is capable of fibril formation},
  number={5},
  volume={75},
  issn={0960-7412},
  journal={The Plant Journal},
  pages={767--780},
  author={Kemen, Eric and Kemen, Ariane and Ehlers, Andreas and Voegele, Ralf T. and Mendgen, Kurt}
}

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Bibliography of Konstanz

Yes