Single‐Molecule Microscopy of the Green Fluorescent Protein Using Simultaneous Two‐Color Excitation
| Type of Publication: | Journal article |
| Publication status: | Published |
| Author: | Jung, Gregor; Wiehler, Jens; Steipe, Boris; Bräuchle, Christoph; Zumbusch, Andreas |
| Year of publication: | 2001 |
| Published in: | ChemPhysChem ; 2 (2001), 6. - pp. 392-396. - ISSN 1439-4235. - eISSN 1439-7641 |
| DOI (citable link): | https://dx.doi.org/10.1002/1439-7641(20010618)2:6<392::AID-CPHC392>3.0.CO;2-7 |
| Summary: |
In vivo microscopy of the Green Fluorescent Protein (GFP), the most important label in cell biology, with single‐molecule sensitivity is hampered by an insufficient signal‐to‐noise ratio. A significant improvement is obtained with a novel two‐color excitation technique. The picture clearly shows the increased brightness of GFP in in vitro single‐molecule assays and in live‐cell microscopy under two‐color illumination (upper cell) as compared to normal illumination (lower cell).
|
| Subject (DDC): | 540 Chemistry |
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JUNG, Gregor, Jens WIEHLER, Boris STEIPE, Christoph BRÄUCHLE, Andreas ZUMBUSCH, 2001. Single‐Molecule Microscopy of the Green Fluorescent Protein Using Simultaneous Two‐Color Excitation. In: ChemPhysChem. 2(6), pp. 392-396. ISSN 1439-4235. eISSN 1439-7641. Available under: doi: 10.1002/1439-7641(20010618)2:6<392::AID-CPHC392>3.0.CO;2-7
@article{Jung2001-06-18Singl-42316, title={Single‐Molecule Microscopy of the Green Fluorescent Protein Using Simultaneous Two‐Color Excitation}, year={2001}, doi={10.1002/1439-7641(20010618)2:6<392::AID-CPHC392>3.0.CO;2-7}, number={6}, volume={2}, issn={1439-4235}, journal={ChemPhysChem}, pages={392--396}, author={Jung, Gregor and Wiehler, Jens and Steipe, Boris and Bräuchle, Christoph and Zumbusch, Andreas} }
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