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Peridinin–chlorophyll–protein reconstituted with chlorophyll mixtures : Preparation, bulk and single molecule spectroscopy

Peridinin–chlorophyll–protein reconstituted with chlorophyll mixtures : Preparation, bulk and single molecule spectroscopy

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BROTOSUDARMO, Tatas H. P., Eckhard HOFMANN, Roger G. HILLER, Stephan WÖRMKE, Sebastian MACKOWSKI, Andreas ZUMBUSCH, Christoph BRÄUCHLE, Hugo SCHEER, 2006. Peridinin–chlorophyll–protein reconstituted with chlorophyll mixtures : Preparation, bulk and single molecule spectroscopy. In: FEBS Letters. 580(22), pp. 5257-5262. ISSN 0014-5793. eISSN 1873-3468. Available under: doi: 10.1016/j.febslet.2006.08.049

@article{Brotosudarmo2006-10-02Perid-42307, title={Peridinin–chlorophyll–protein reconstituted with chlorophyll mixtures : Preparation, bulk and single molecule spectroscopy}, year={2006}, doi={10.1016/j.febslet.2006.08.049}, number={22}, volume={580}, issn={0014-5793}, journal={FEBS Letters}, pages={5257--5262}, author={Brotosudarmo, Tatas H. P. and Hofmann, Eckhard and Hiller, Roger G. and Wörmke, Stephan and Mackowski, Sebastian and Zumbusch, Andreas and Bräuchle, Christoph and Scheer, Hugo} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/42307"> <dc:rights>terms-of-use</dc:rights> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-05-11T12:17:35Z</dcterms:available> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:creator>Hiller, Roger G.</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Hiller, Roger G.</dc:contributor> <dcterms:issued>2006-10-02</dcterms:issued> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-05-11T12:17:35Z</dc:date> <dc:contributor>Scheer, Hugo</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:contributor>Brotosudarmo, Tatas H. P.</dc:contributor> <dc:creator>Mackowski, Sebastian</dc:creator> <dc:contributor>Zumbusch, Andreas</dc:contributor> <dcterms:title>Peridinin–chlorophyll–protein reconstituted with chlorophyll mixtures : Preparation, bulk and single molecule spectroscopy</dcterms:title> <dc:creator>Scheer, Hugo</dc:creator> <dc:contributor>Mackowski, Sebastian</dc:contributor> <dc:contributor>Wörmke, Stephan</dc:contributor> <dcterms:abstract xml:lang="eng">Reconstitution of the 16 kDa N-terminal domain of the peridinin-chlorophyll-protein, N-PCP, with mixtures of chlorophyll a (Chl a) and Chl b, resulted in 32 kDa complexes containing two pigment clusters, each bound to one N-PCP. Besides homo-chlorophyllous complexes, hetero-chlorophyllous ones were obtained that contain Chl a in one pigment cluster, and Chl b in the other. Binding of Chl b is stronger than that of the native pigment, Chl a. Energy transfer from Chl b to Chl a is efficient, but there are only weak interactions between the two pigments. Individual homo- and hetero-chlorophyllous complexes were investigated by single molecule spectroscopy using excitation into the peridinin absorption band and scanning of the Chl fluorescence, the latter show frequently well resolved emissions of the two pigments.</dcterms:abstract> <dc:creator>Wörmke, Stephan</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/42307"/> <dc:creator>Brotosudarmo, Tatas H. P.</dc:creator> <dc:contributor>Bräuchle, Christoph</dc:contributor> <dc:creator>Zumbusch, Andreas</dc:creator> <dc:creator>Bräuchle, Christoph</dc:creator> <dc:contributor>Hofmann, Eckhard</dc:contributor> <dc:creator>Hofmann, Eckhard</dc:creator> </rdf:Description> </rdf:RDF>

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