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Apoptosis and expression of apoptosis-related genes in mouse intestinal tissue after whole-body proton exposure

Apoptosis and expression of apoptosis-related genes in mouse intestinal tissue after whole-body proton exposure

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PURGASON, Ashley, Ye ZHANG, Stanley R. HAMILTON, Daila S. GRIDLEY, Ayodotun SODIPE, Olufisayo JEJELOWO, Govindarajan RAMESH, Maria MORENO-VILLANUEVA, Honglu WU, 2018. Apoptosis and expression of apoptosis-related genes in mouse intestinal tissue after whole-body proton exposure. In: Molecular and Cellular Biochemistry. 442(1-2), pp. 155-168. ISSN 0300-8177. eISSN 1573-4919. Available under: doi: 10.1007/s11010-017-3200-0

@article{Purgason2018-05Apopt-42306, title={Apoptosis and expression of apoptosis-related genes in mouse intestinal tissue after whole-body proton exposure}, year={2018}, doi={10.1007/s11010-017-3200-0}, number={1-2}, volume={442}, issn={0300-8177}, journal={Molecular and Cellular Biochemistry}, pages={155--168}, author={Purgason, Ashley and Zhang, Ye and Hamilton, Stanley R. and Gridley, Daila S. and Sodipe, Ayodotun and Jejelowo, Olufisayo and Ramesh, Govindarajan and Moreno-Villanueva, Maria and Wu, Honglu} }

Sodipe, Ayodotun Jejelowo, Olufisayo 2018-05-11T09:31:21Z Sodipe, Ayodotun Moreno-Villanueva, Maria Hamilton, Stanley R. Purgason, Ashley Wu, Honglu Jejelowo, Olufisayo Moreno-Villanueva, Maria eng Wu, Honglu Zhang, Ye Hamilton, Stanley R. Gridley, Daila S. 2018-05 Energetic protons are the most abundant particle type in space and can pose serious health risks to astronauts during long-duration missions. The health effects of proton exposure are also a concern for cancer patients undergoing radiation treatment with accelerated protons. To investigate the damage induced by energetic protons in vivo to radiosensitive organs, 6-week-old BALB/c male mice were subjected to 250 MeV proton radiation at whole-body doses of 0.1, 1, and 2 Gy. The gastrointestinal (GI) tract of each exposed animal was dissected 4 h post-irradiation, and the isolated small intestinal tissue was analyzed for histopathological and gene expression changes. Histopathologic observation of the tissue using standard hematoxylin and eosin (H&E) staining methods to screen for morphologic changes showed a marked increase in apoptotic lesions for even the lowest dose of 0.1 Gy, similar to X- or γ rays. The percentage of apoptotic cells increased dose-dependently, but the dose response appeared supralinear, indicating hypersensitivity at low doses. A significant decrease in surviving crypts and mucosal surface area, as well as in cell proliferation, was also observed in irradiated mice. Gene expression analysis of 84 genes involved in the apoptotic process showed that most of the genes affected by protons were common between the low (0.1 Gy) and high (1 and 2 Gy) doses. However, the genes that were distinctively responsive to the low or high doses suggest that high doses of protons may cause apoptosis in the small intestine by direct damage to the DNA, whereas low doses of protons may trigger apoptosis through a different stress response mechanism. Zhang, Ye 2018-05-11T09:31:21Z Ramesh, Govindarajan Purgason, Ashley Ramesh, Govindarajan Gridley, Daila S. Apoptosis and expression of apoptosis-related genes in mouse intestinal tissue after whole-body proton exposure

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