Type of Publication: | Journal article |
Publication status: | Published |
Author: | Butz, Karin; Ristriani, Tutik; Hengstermann, Arnd; Denk, Claudia; Scheffner, Martin; Hoppe-Seyler, Felix |
Year of publication: | 2003 |
Published in: | Oncogene ; 22 (2003), 38. - pp. 5938-5945. - ISSN 0950-9232. - eISSN 1476-5594 |
Pubmed ID: | 12955072 |
DOI (citable link): | https://dx.doi.org/10.1038/sj.onc.1206894 |
Summary: |
The targeted inhibition of antiapoptotic factors in tumour cells may provide a rational approach towards the development of novel anticancer therapies. Using human papillomavirus (HPV)-transformed cells as a model system, we investigated if RNA interference (RNAi)-mediated gene silencing can be employed in order to overcome the apoptosis resistance of cancer cells. We found that both vector-borne and synthetic small interfering (si)RNAs, specifically directed against the antiapoptotic HPV E6 oncogene, restored dormant tumour suppressor pathways in HPV-positive cancer cells that are otherwise inactive in the presence of E6. This ultimately resulted in massive apoptotic cell death, selectively in HPV-positive tumour cells. These findings show that RNAi provides a powerful molecular strategy to inactivate intracellular E6 function efficiently. Moreover, they define E6 as a most promising therapeutic target to eliminate HPV-positive tumour cells specifically by RNAi. Thus, by sequence-specific targeting of antiapoptotic genes, siRNAs may be developed into novel therapeutics that can efficiently correct the apoptosis deficiency of cancer cells.
|
Subject (DDC): | 570 Biosciences, Biology |
Keywords: | RNA interference; human papillomavirus; apoptosis; tumour virus; tumour therapy |
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BUTZ, Karin, Tutik RISTRIANI, Arnd HENGSTERMANN, Claudia DENK, Martin SCHEFFNER, Felix HOPPE-SEYLER, 2003. siRNA targeting of the viral E6 oncogene efficiently kills human papillomavirus-positive cancer cells. In: Oncogene. 22(38), pp. 5938-5945. ISSN 0950-9232. eISSN 1476-5594. Available under: doi: 10.1038/sj.onc.1206894
@article{Butz2003-09-04siRNA-42200, title={siRNA targeting of the viral E6 oncogene efficiently kills human papillomavirus-positive cancer cells}, year={2003}, doi={10.1038/sj.onc.1206894}, number={38}, volume={22}, issn={0950-9232}, journal={Oncogene}, pages={5938--5945}, author={Butz, Karin and Ristriani, Tutik and Hengstermann, Arnd and Denk, Claudia and Scheffner, Martin and Hoppe-Seyler, Felix} }
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