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Crystallization and preliminary X-ray analysis of the coiled-coil domain of dystrophia myotonica kinase

Crystallization and preliminary X-ray analysis of the coiled-coil domain of dystrophia myotonica kinase

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GARCIA, Pilar, Marco MARINO, Olga MAYANS, 2004. Crystallization and preliminary X-ray analysis of the coiled-coil domain of dystrophia myotonica kinase. In: Acta crystallographica Section D : Biological Crystallography. 60(12 - 2), pp. 2336-2339. ISSN 0907-4449. eISSN 1399-0047. Available under: doi: 10.1107/S0907444904026873

@article{Garcia2004Cryst-42088, title={Crystallization and preliminary X-ray analysis of the coiled-coil domain of dystrophia myotonica kinase}, year={2004}, doi={10.1107/S0907444904026873}, number={12 - 2}, volume={60}, issn={0907-4449}, journal={Acta crystallographica Section D : Biological Crystallography}, pages={2336--2339}, author={Garcia, Pilar and Marino, Marco and Mayans, Olga} }

Garcia, Pilar The coiled-coil domain of dystrophia myotonica protein kinase (DMPK) has been cloned, overexpressed, purified and crystallized. Two crystal forms have been obtained that belong to space groups P3 and P2<sub>(1)</sub>2<sub>(1)</sub>2<sub>(1)</sub> and diffract to 2.4 and 1.6 A resolution, respectively. Experimental phases were obtained by MAD from an SeMet derivative. The location of selenium sites used molecular-replacement phases obtained from search models lacking sequence similarity with the coiled-coil under study. Both crystal forms contain three polypeptide chains in the asymmetric unit. eng 2018-04-18T10:16:03Z Marino, Marco Marino, Marco 2018-04-18T10:16:03Z Crystallization and preliminary X-ray analysis of the coiled-coil domain of dystrophia myotonica kinase Mayans, Olga Garcia, Pilar 2004 Mayans, Olga

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