Site-specific PEGylation of proteins containing unnatural amino acids

Deiters, Alexander; Cropp, T. Ashton; Summerer, Daniel; Mukherji, Mridul; Schultz, Peter G.

Site-specific PEGylation of proteins containing unnatural amino acids

Files

Summerer.pdf(8.41 MB)

Date

2004

Authors

Deiters, Alexander

Cropp, T. Ashton

Summerer, Daniel

Mukherji, Mridul

Schultz, Peter G.

Publication type

Journal article

Published in

Bioorganic & Medicinal Chemistry Letters ; 14 (2004), 23. - pp. 5743-5745. - ISSN 0960-894X

Abstract

Here, we report a generally applicable PEGylation methodology based on the site-specific incorporation of para-azidophenylalanine into proteins in yeast. The azido group was used in a mild [3+2] cycloaddition reaction with an alkyne derivatized PEG reagent to afford selectively PEGylated protein. This strategy should be useful for the generation of selectively PEGylated proteins for therapeutic applications.

Subject (DDC)

540 Chemistry

Cite This

ISO 690

DEITERS, Alexander, T. Ashton CROPP, Daniel SUMMERER, Mridul MUKHERJI, Peter G. SCHULTZ, 2004. Site-specific PEGylation of proteins containing unnatural amino acids. In: Bioorganic & Medicinal Chemistry Letters. 14(23), pp. 5743-5745. ISSN 0960-894X. Available under: doi: 10.1016/j.bmcl.2004.09.059

BibTex

@article{Deiters2004-12-06Sites-419,
  year={2004},
  doi={10.1016/j.bmcl.2004.09.059},
  title={Site-specific PEGylation of proteins containing unnatural amino acids},
  number={23},
  volume={14},
  issn={0960-894X},
  journal={Bioorganic & Medicinal Chemistry Letters},
  pages={5743--5745},
  author={Deiters, Alexander and Cropp, T. Ashton and Summerer, Daniel and Mukherji, Mridul and Schultz, Peter G.}
}

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