Type of Publication: | Journal article |
Publication status: | Published |
Author: | Geitner, Anne-Juliane; Weininger, Ulrich; Paulsen, Hauke; Balbach, Jochen; Kovermann, Michael |
Year of publication: | 2017 |
Published in: | Biochemistry ; 56 (2017), 50. - pp. 6533-6543. - ISSN 0006-2960. - eISSN 1520-4995 |
Pubmed ID: | 29155566 |
DOI (citable link): | https://dx.doi.org/10.1021/acs.biochem.7b00786 |
Summary: |
SlpA (SlyD-like protein A) comprises two domains, a FK506 binding domain (FKBP fold) of moderate prolyl cis/trans-isomerase activity and an inserted in flap (IF) domain that hosts its chaperone activity. Here we present the nuclear magnetic resonance (NMR) solution structure of apo Escherichia coli SlpA determined by NMR that mirrors the structural properties seen for various SlyD homologues. Crucial structural differences in side-chain orientation arise for F37, which points directly into the hydrophobic core of the active site. It forms a prominent aromatic stacking with F15, one of the key residues for PPIase activity, thus giving a possible explanation for the inherently low PPIase activity of SlpA. The IF domain reveals the highest stability within the FKBP-IF protein family, most likely arising from an aromatic cluster formed by four phenylalanine residues. Both the thermodynamic stability and the PPIase and chaperone activity let us speculate that SlpA is a backup system for homologous bacterial systems under unfavorable conditions.
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Subject (DDC): | 540 Chemistry |
Bibliography of Konstanz: | Yes |
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GEITNER, Anne-Juliane, Ulrich WEININGER, Hauke PAULSEN, Jochen BALBACH, Michael KOVERMANN, 2017. Structure-Based Insights into the Dynamics and Function of Two-Domain SlpA from Escherichia coli. In: Biochemistry. 56(50), pp. 6533-6543. ISSN 0006-2960. eISSN 1520-4995. Available under: doi: 10.1021/acs.biochem.7b00786
@article{Geitner2017-12-19Struc-41128, title={Structure-Based Insights into the Dynamics and Function of Two-Domain SlpA from Escherichia coli}, year={2017}, doi={10.1021/acs.biochem.7b00786}, number={50}, volume={56}, issn={0006-2960}, journal={Biochemistry}, pages={6533--6543}, author={Geitner, Anne-Juliane and Weininger, Ulrich and Paulsen, Hauke and Balbach, Jochen and Kovermann, Michael} }
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