New Animal-free Concepts and Test Methods for Developmental Toxicity and Peripheral Neurotoxicity

Leist, Marcel

New Animal-free Concepts and Test Methods for Developmental Toxicity and Peripheral Neurotoxicity

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Date

2017

Authors

Leist, Marcel

EU project number

681002

Project

EUToxRisk21

Collections

Biologie

Publication type

Journal article

Publication status

Published

Published in

Alternatives to Laboratory Animals : ATLA ; 45 (2017), 5. - pp. 253-260. - ISSN 0261-1929

Abstract

The complex toxicological fields of repeat dose organ toxicity (RDT) and developmental and reproductive toxicity (DART) still require new concepts and approaches to achieve a fully animal-free safety assessment of chemicals. One novel approach is the generation of relevant human cell types from pluripotent stem cells, and the use of such cells for the establishment of phenotypic test methods. Due to their broad endpoints, such tests capture multiple types of toxicants, i.e. they are a readout for the activation of many adverse outcome pathways (AOPs). The 2016 Lush Science Prize was awarded for the development of one such assay, the PeriTox test, which uses human peripheral neurons generated from stem cells. The assay endpoints measure various cell functions, and these give information on the potential neurotoxicity and developmental neurotoxicity hazard of test compounds. The PeriTox test method has a high predictivity and sensitivity for peripheral neurotoxicants, and thus addresses the inherent challenges in pesticide testing and drug development. Data from the test can be obtained quickly and at a relatively high-throughput, and thus, the assay has the potential to replace animal-based safety assessment during early product development or for screening potential environmental toxicants.

Subject (DDC)

570 Biosciences, Biology

Keywords

developmental toxicity, high-content imaging, human stem cells, neurite growth, peripheral neuropathy, replacement

Cite This

ISO 690

LEIST, Marcel, 2017. New Animal-free Concepts and Test Methods for Developmental Toxicity and Peripheral Neurotoxicity. In: Alternatives to Laboratory Animals : ATLA. 45(5), pp. 253-260. ISSN 0261-1929

BibTex

@article{Leist2017Anima-40958,
  year={2017},
  title={New Animal-free Concepts and Test Methods for Developmental Toxicity and Peripheral Neurotoxicity},
  url={http://www.atla.org.uk/new-animal-free-concepts-and-test-methods-for-developmental-toxicity-and-peripheral-neurotoxicity/},
  number={5},
  volume={45},
  issn={0261-1929},
  journal={Alternatives to Laboratory Animals : ATLA},
  pages={253--260},
  author={Leist, Marcel}
}

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URL of original publication

http://www.atla.org.uk/new-animal-free-concepts-and-test-methods-for-developmental-toxicity-and-peripheral-neurotoxicity/

Test date of URL

2018-05-09

Bibliography of Konstanz

Yes