Characterizing Host Receptor Recognition by Individual Bacterial Pathogens


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KUESPERT, Katharina, Christof R. HAUCK, 2009. Characterizing Host Receptor Recognition by Individual Bacterial Pathogens. In: RUPP, Steffen, ed. and others. Host-pathogen interactions : methods and protocols. Totowa, NJ:Humana Press, pp. 57-65. ISBN 978-1-58829-886-7. Available under: doi: 10.1007/978-1-59745-204-5_5

@incollection{Kuespert2009Chara-40582, title={Characterizing Host Receptor Recognition by Individual Bacterial Pathogens}, year={2009}, doi={10.1007/978-1-59745-204-5_5}, number={470}, isbn={978-1-58829-886-7}, address={Totowa, NJ}, publisher={Humana Press}, series={Methods in Molecular Biology}, booktitle={Host-pathogen interactions : methods and protocols}, pages={57--65}, editor={Rupp, Steffen}, author={Kuespert, Katharina and Hauck, Christof R.} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:contributor>Hauck, Christof R.</dc:contributor> <dcterms:issued>2009</dcterms:issued> <dc:date rdf:datatype="">2017-11-13T08:31:35Z</dc:date> <dc:creator>Hauck, Christof R.</dc:creator> <dc:contributor>Kuespert, Katharina</dc:contributor> <bibo:uri rdf:resource=""/> <dc:creator>Kuespert, Katharina</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:isPartOf rdf:resource=""/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:available rdf:datatype="">2017-11-13T08:31:35Z</dcterms:available> <dspace:isPartOfCollection rdf:resource=""/> <dc:language>eng</dc:language> <dcterms:abstract xml:lang="eng">A critical determinant of host range and specificity relies on the ability of pathogenic bacteria to recognize eukaryotic cell surface molecules via specialized adhesins. The specific adhesin-receptor interaction allows pathogens to tightly bind to their target cells, thereby facilitating the colonization of host tissues. Therefore, the identification and characterization of bacterial adhesins is a major topic in infection biology. This chapter focuses on a rapid and simple method for the analysis of adhesin-receptor interactions that permits the characterization of receptor binding properties at the level of single bacteria. Accordingly, this methodological approach is ideally suited for the analysis of adhesins expressed in a phase-variable manner and for the study of heterogeneous bacterial populations. Besides focusing on the receptor-binding assay, this chapter describes the production of fluorescence-tagged soluble host receptor domains required for conducting this assay.</dcterms:abstract> <dcterms:title>Characterizing Host Receptor Recognition by Individual Bacterial Pathogens</dcterms:title> </rdf:Description> </rdf:RDF>

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