Simultaneous IR-spectroscopic observation of α-synuclein, lipids, and solvent reveals an alternative membrane-induced oligomerization pathway
| Type of Publication: | Journal article |
| Publication status: | Published |
| URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-2-1eodq8f5g6c7y3 |
| Author: | Fallah, Mohammad A; Gerding, Hanne R.; Scheibe, Christian; Drescher, Malte; Karreman, Christiaan; Schildknecht, Stefan; Leist, Marcel; Hauser, Karin |
| Year of publication: | 2017 |
| Published in: | ChemBioChem ; 18 (2017), 23. - pp. 2312-2316. - ISSN 1439-4227. - eISSN 1439-7633 |
| Pubmed ID: | 28980756 |
| DOI (citable link): | https://dx.doi.org/10.1002/cbic.201700355 |
| Summary: |
The intrinsically disordered protein α-synuclein (αS), a known pathogenic factor for Parkinson's disease, can adopt defined secondary structures when interacting with membranes or during fibrillation. The αS-lipid interaction and the implications of this process for aggregation and damage to membranes are still poorly understood. Therefore, we established a label-free infrared (IR) spectroscopic approach to simultaneously monitor αS conformation and membrane integrity. IR showed its unique sensitivity for identifying distinct α-structured aggregates. A comparative study of wildtype αS and the naturally occurring splicing variant αS Δexon3 yielded new insights into the membrane's capability of altering aggregation pathways.
|
| Subject (DDC): | 570 Biosciences, Biology |
| Link to License: | In Copyright |
| Bibliography of Konstanz: | Yes |
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FALLAH, Mohammad A, Hanne R. GERDING, Christian SCHEIBE, Malte DRESCHER, Christiaan KARREMAN, Stefan SCHILDKNECHT, Marcel LEIST, Karin HAUSER, 2017. Simultaneous IR-spectroscopic observation of α-synuclein, lipids, and solvent reveals an alternative membrane-induced oligomerization pathway. In: ChemBioChem. 18(23), pp. 2312-2316. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201700355
@article{Fallah2017-12-05Simul-40331, title={Simultaneous IR-spectroscopic observation of α-synuclein, lipids, and solvent reveals an alternative membrane-induced oligomerization pathway}, year={2017}, doi={10.1002/cbic.201700355}, number={23}, volume={18}, issn={1439-4227}, journal={ChemBioChem}, pages={2312--2316}, author={Fallah, Mohammad A and Gerding, Hanne R. and Scheibe, Christian and Drescher, Malte and Karreman, Christiaan and Schildknecht, Stefan and Leist, Marcel and Hauser, Karin} }
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