Aufgrund von Vorbereitungen auf eine neue Version von KOPS, können am Montag, 6.2. und Dienstag, 7.2. keine Publikationen eingereicht werden. (Due to preparations for a new version of KOPS, no publications can be submitted on Monday, Feb. 6 and Tuesday, Feb. 7.)
Type of Publication: | Journal article |
Publication status: | Published |
Author: | Brausemann, Anton; Gemmecker, Sandra; Koschmieder, Julian; Ghisla, Sandro; Beyer, Peter; Einsle, Oliver |
Year of publication: | 2017 |
Published in: | Structure ; 25 (2017), 8. - pp. 1222-1232. - ISSN 0969-2126. - eISSN 1878-4186 |
Pubmed ID: | 28669634 |
DOI (citable link): | https://dx.doi.org/10.1016/j.str.2017.06.002 |
Summary: |
Cyanobacteria and plants synthesize carotenoids via a poly-cis pathway starting with phytoene, a membrane-bound C40 hydrocarbon. Phytoene desaturase (PDS) introduces two double bonds and concomitantly isomerizes two neighboring double bonds from trans to cis. PDS assembles into homo-tetramers that interact monotopically with membranes. A long hydrophobic tunnel is proposed to function in the sequential binding of phytoene and the electron acceptor plastoquinone. The herbicidal inhibitor norflurazon binds at a plastoquinone site thereby blocking reoxidation of FADred. Comparison with the sequence-dissimilar bacterial carotene desaturase CRTI reveals substantial similarities in the overall protein fold, defining both as members of the GR2 family of flavoproteins. However, the PDS active center architecture is unprecedented: no functional groups are found in the immediate flavin vicinity that might participate in dehydrogenation and isomerization. This suggests that the isoalloxazine moiety is sufficient for catalysis. Despite mechanistic differences, an ancient evolutionary relation of PDS and CRTI is apparent.
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Subject (DDC): | 570 Biosciences, Biology |
Comment on publication: | Anhang auf den Seiten e1-e3 |
Bibliography of Konstanz: | Yes |
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BRAUSEMANN, Anton, Sandra GEMMECKER, Julian KOSCHMIEDER, Sandro GHISLA, Peter BEYER, Oliver EINSLE, 2017. Structure of Phytoene Desaturase Provides Insights into Herbicide Binding and Reaction Mechanisms Involved in Carotene Desaturation. In: Structure. 25(8), pp. 1222-1232. ISSN 0969-2126. eISSN 1878-4186. Available under: doi: 10.1016/j.str.2017.06.002
@article{Brausemann2017-08Struc-40059, title={Structure of Phytoene Desaturase Provides Insights into Herbicide Binding and Reaction Mechanisms Involved in Carotene Desaturation}, year={2017}, doi={10.1016/j.str.2017.06.002}, number={8}, volume={25}, issn={0969-2126}, journal={Structure}, pages={1222--1232}, author={Brausemann, Anton and Gemmecker, Sandra and Koschmieder, Julian and Ghisla, Sandro and Beyer, Peter and Einsle, Oliver}, note={Anhang auf den Seiten e1-e3} }
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