Experimental Verification of an Exact Evanescent Light Scattering Model for TIRM

Zitieren

Dateien zu dieser Ressource

Dateien Größe Format Anzeige

Zu diesem Dokument gibt es keine Dateien.

HERTLEIN, Christopher, Norbert RIEFLER, Elena EREMINA, Thomas WRIEDT, Yuri EREMIN, Laurent HELDEN, Clemens BECHINGER, 2008. Experimental Verification of an Exact Evanescent Light Scattering Model for TIRM. In: Langmuir. 24(1), pp. 1-4. ISSN 0743-7463. eISSN 1520-5827

@article{Hertlein2008-01Exper-39565, title={Experimental Verification of an Exact Evanescent Light Scattering Model for TIRM}, year={2008}, doi={10.1021/la703322d}, number={1}, volume={24}, issn={0743-7463}, journal={Langmuir}, pages={1--4}, author={Hertlein, Christopher and Riefler, Norbert and Eremina, Elena and Wriedt, Thomas and Eremin, Yuri and Helden, Laurent and Bechinger, Clemens} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/39565"> <dcterms:issued>2008-01</dcterms:issued> <dc:creator>Eremina, Elena</dc:creator> <dc:language>eng</dc:language> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-07-12T11:32:09Z</dc:date> <dc:contributor>Bechinger, Clemens</dc:contributor> <dcterms:abstract xml:lang="eng">Total internal reflection microscopy (TIRM) is a method for the precise measurement of interaction potentials between a spherical colloidal particle and a wall. The method is based on single-particle evanescent wave light scattering. The well-established model used to interpret TIRM data is based on an exponential relation between scattering intensity and particle wall distance. However, applying this model for a certain range of experimental parameters leads to significant distortions of the measured potentials. Using a TIRM setup based on a two-wavelength illumination technique, we were able to directly measure the intensity distance relation revealing deviations from an exponential decay. The intensity-distance relations could be compared to scattering simulations taking into account exact experimental parameters and multiple reflections between a particle and the wall. Converging simulation results were independently obtained by the T-matrix method and the discrete sources method (DSM) and show excellent agreement with experiments. Using the new scattering model for data evaluation, we could reconstruct the correct potential shape for distorted interaction potentials as we demonstrate. The comparison of simulations to experiment intrinsically yields a new method to determine absolute particle-wall distances, a highly desired quantity in TIRM experiments.</dcterms:abstract> <dc:contributor>Eremin, Yuri</dc:contributor> <dc:creator>Helden, Laurent</dc:creator> <dc:creator>Riefler, Norbert</dc:creator> <dc:creator>Eremin, Yuri</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-07-12T11:32:09Z</dcterms:available> <dc:creator>Bechinger, Clemens</dc:creator> <dc:creator>Hertlein, Christopher</dc:creator> <dcterms:title>Experimental Verification of an Exact Evanescent Light Scattering Model for TIRM</dcterms:title> <dc:contributor>Eremina, Elena</dc:contributor> <dc:contributor>Helden, Laurent</dc:contributor> <dc:creator>Wriedt, Thomas</dc:creator> <dc:contributor>Riefler, Norbert</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/39565"/> <dc:contributor>Hertlein, Christopher</dc:contributor> <dc:contributor>Wriedt, Thomas</dc:contributor> </rdf:Description> </rdf:RDF>

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto